Preparation and evaluation of wound healing activity of phytosomes of crocetin from Nyctanthes arbor-tristis in rats

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2022-07-01 DOI:10.1016/j.jtcme.2021.10.002

Manasi Varadkar, Chhaya Gadgoli

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引用次数: 4

Abstract

Background and aim

Orange colored tubular calyx of the flowers of Nyctanthes arbor-tristis contains an apocarotenoid crocin as a major ingredient, which is originally detected to be major colouring principle of saffron stigma. Saffron stigma exhibits good wound healing activity owing to the presence of crocin. The present study is aimed at isolation of crocetin, from tubular calyx of N. arbortristis and improve stability through entrapping in vesicles followed by evaluation of wound healing activity of the topical formulation thereof.

Experimental procedure

Crocetin was isolated by treating ethanolic extract of tubular calyx of N. arbor-tristis with sodium bicarbonate followed by regeneration of crocetin using hydrochloric acid. The phytosomes were prepared by lipid film hydration technique. The gel containing phytosomes equivalent to crocetin 1% w/w, was then evaluated for wound healing activity through applications on incision and excision wounds inflicted in Wister albino rats.

Results

Stability of crocetin was found to be increased due to entrapment into phytosomes. The studies revealed that both types of wounds upon treatment with gel containing crocetin phytosomes, indicated good wound healing potential, as the epithelization period was significantly (P < 0.001)decreased as compared to the control group from 26 to 9 days, in excision wound model and significant (P < 0.001)increase in breaking strength of repaired skin, as compared to control from 328.8 to 857.0 gm in incision wound model.

Conclusion

Crocetin from tubular calyx of Nyctanthes arbor-tristis indicated to be potential wound healing phytoconstituent.

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大鼠三倍体中番红花素植物体的制备及对伤口愈合活性的评价

背景与目的桔黄色柱状花的管状肾盏中含有一种类胡萝卜素番红花苷作为主要成分，该成分最初被认为是藏红花柱头的主要着色原理。藏红花柱头由于番红花苷的存在而表现出良好的伤口愈合活性。本研究旨在从长春藤的管状肾盏中分离番红花苷，并通过包埋在囊泡中提高稳定性，然后评估其局部制剂的伤口愈合活性。实验方法用碳酸氢钠处理三叶草肾小管的乙醇提取物，再用盐酸再生番红花素，分离番红花素。采用脂质膜水合技术制备了植物体。然后，通过应用于Wister白化大鼠的切口和切除伤口，评估含有相当于西红花素1%w/w的植物体的凝胶的伤口愈合活性。结果番红花素包埋在植物体中，稳定性提高。研究表明，在切除伤口模型中，两种类型的伤口在用含有番红花素植物体的凝胶治疗后都显示出良好的伤口愈合潜力，因为与对照组相比，上皮化期从26天到9天显著缩短（P<；0.001），并且修复皮肤的断裂强度显著增加（P<），与切开伤口模型中328.8至857.0gm的对照组相比。结论柱花草管盏中的番红花素是一种潜在的创伤愈合植物成分。

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