Detam 1 black soybean against cisplatin-induced acute ren failure on rat model via antioxidant, antiinflammatory and antiapoptosis potential

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2022-07-01 DOI:10.1016/j.jtcme.2022.01.004

Wahyu Widowati , Sijani Prahastuti , Meilinah Hidayat , Stella Tinia Hasianna , Roro Wahyudianingsih , The Fransiska Eltania , Alya Mardhotillah Azizah , Jenifer Kiem Aviani , Mawar Subangkit , Rr Anisa Siwianti Handayani , Hanna Sari Widya Kusuma

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引用次数: 7

Abstract

Background and aim

Cis-Diamminedichloroplatinum (II) (Cisplatin) is one of the most synthetic anticancer drug but have several adverse effects and one of them is acute ren failure. Cisplatin can induce nephrotoxicity occur via the toxic generation of reactive oxygen species (ROS). Black soybean (Glycine max L. Merr.) has been reported contain high levels of phenolics and anthocyanins that has antioxidant activity. This study aims to determine the effect of ethanol extract of black soybean (EEBS) against cisplatin-induced nephrotoxicity in rats.

Experimental procedure

Cisplatin-induced nephrotoxicity rats treated with EEBS and the blood samples taken on days 0, 9, and 18. The effects of EEBS was evaluated by determining Interferon-γ (IFN-γ), Caspase-3 (Casp-3), and Interleukin-1β (IL-1β) expression using immunohistochemistry (IHC), blood urea nitrogen (BUN), Uric Acid (UA) content and catalase (CAT) content in the blood plasma with colorimetric assay kit.

Results and conclusion

Based on the results, EEBS treatment had successfully reduced pro-inflammatory cytokines IL-1β and IFN-γ, and improved physiological condition by lowering BUN and UA content while increasing CAT activity. No significant effect was found in Casp-3 expression. EEBS has potential to improve acute renal failure condition through inflammatory suppression and renal function improvement.

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Detam 1黑豆通过抗氧化、抗炎和抗凋亡作用对抗顺铂诱导的大鼠急性肾功能衰竭

背景与目的二胺二氯铂（II）（顺铂）是合成量最大的抗癌药物之一，但有多种不良反应，其中一种是急性肾功能衰竭。顺铂可通过活性氧（ROS）的毒性产生诱导肾毒性。据报道，黑豆（Glycine max L.Merr.）含有高水平的酚类和花青素，具有抗氧化活性。本研究旨在确定黑豆乙醇提取物（EEBS）对顺铂诱导的大鼠肾毒性的影响。实验程序用EEBS处理的顺铂诱导的肾毒性大鼠和在第0、9和18天采集的血样。采用免疫组织化学（IHC）、血尿素氮（BUN）、尿酸（UA）含量和过氧化氢酶（CAT）含量测定血浆中干扰素-γ（IFN-γ）、半胱氨酸蛋白酶-3（Casp-3）和白细胞介素-1β（IL-1β。结果与结论EEBS治疗成功地降低了促炎细胞因子IL-1β和IFN-γ，并通过降低BUN和UA含量同时提高CAT活性来改善生理状况。Casp-3的表达没有发现显著的影响。EEBS具有通过抑制炎症和改善肾功能来改善急性肾功能衰竭的潜力。

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