A Novel GC-MS Bioanalytical Method for Natural Borneol and Its Application in Investigating Natural Borneol Distribution in Mice

World Science and Technology Pub Date : 2009-12-01 DOI:10.1016/S1876-3553(10)60038-5

Huang Ping , Jiang Xiaofei , Zou Jiali , Yuan Yuemei , Yao Meicun , Lu Yasong

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引用次数: 7

Abstract

One of the major goals of our group is to understand the meridian tropism of natural borneol (NB). As a first step, this study served two purposes: to develop a GC-MS bioanalytical method for NB, and with the aid of this method to investigate NB distribution in mice. Mouse tissue samples, spiked with naphthalene as internal standard, were homogenated with NS and extracted using hexane. The extract supernatant was analyzed in GC-MS with conditions of DB-5MS capillary column (0.25mm×30m×0.25μm), electron impact (EI) ionization with energy of 70 eV and multiplier voltage of 1100 V, and Selected Ion Monitor (SIM, m/z 95 for camphor and NB, whereas m/z 128 for naphthalene) detection mode. The calibration curve was linear in the range from 22 ng/mL to 22 μg/mL with a regression equation Y= 0.000486X + 0.00036, R²=0.999 (n=3). The limit of detection was 10 ng/mL. The lower limit of quantitation was 22 ng/mL. The within-day and inter-day precisions were 3.72∼6.11% and 7.29∼17.6%, respectively. The relative recovery was 85.95∼107.93% and extraction recovery was 93.04∼103.03%. This bioanalytical method has sufficient recovery and is accurate and reliable for quantitating NB in tissues. The tissue distribution up to 150 min after a single oral dose of NB was investigated in mice. This compound was detected in most tissues. The liver had the highest concentration, followed by kidney, heart, brain, and others. Tissue concentration-time curves had double or triple peaks. The tissue distribution pattern suggested that NB may attribute to the cardio-system.

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天然冰片的GC-MS生物分析新方法及其在小鼠体内分布研究中的应用

我们小组的主要目标之一是了解天然龙脑（NB）的归经性。作为第一步，本研究有两个目的：开发NB的GC-MS生物分析方法，并借助该方法研究NB在小鼠中的分布。用萘作为内标物掺入的小鼠组织样品用NS匀浆并用己烷提取。在DB-5MS毛细管柱（0.25mm×30m×0.25μm）、能量为70eV、倍增电压为1100V的电子碰撞（EI）电离和选择性离子监测器（SIM，樟脑和NB为m/z95，萘为m/z128）检测模式下，用GC-MS分析提取物上清液。校准曲线在22 ng/mL至22μg/mL范围内呈线性，回归方程Y=0.000486X+0.00036，R2=0.999（n=3）。检测限为10ng/mL。定量下限为22 ng/mL。日内和日间精密度分别为3.72～6.11%和7.29～17.6%。相对回收率为85.95～107.93%，提取回收率为93.04～103.03%。该生物分析方法具有足够的回收率，用于定量组织中的NB是准确可靠的。在小鼠中研究单次口服NB剂量后长达150分钟的组织分布。这种化合物在大多数组织中都被检测到。肝脏的浓度最高，其次是肾脏、心脏、大脑和其他器官。组织浓度-时间曲线具有双峰或三峰。组织分布模式提示NB可能与心血管系统有关。

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World Science and Technology

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