In vitro anti-HSV-2 activity and mechanism of action of proanthocyanidin A-1 from Vaccinium vitis-idaea

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2004-10-15 DOI:10.1002/jsfa.1958

Hua-Yew Cheng, Ta-Chen Lin, Chien-Min Yang, Den-En Shieh, Chun-Ching Lin

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引用次数: 38

Abstract

The aim of this study was to investigate the in vitro anti-HSV-2 activity and mechanism of action of proanthocyanidin A-1, a compound isolated from Vaccinium vitis-idaea Linn (Ericaceae). The results demonstrated that proanthocyanidin A-1 exhibited anti-HSV-2 activity. The IC₅₀ value for the XTT assay was 73.3 ± 14.5 µM and the IC₅₀ and IC₉₀ values for the plaque reduction assay were 41.9 ± 2.0 and 62.8 ± 6.3 µM respectively. Proanthocyanidin A-1 showed no cell cytotoxic effect at concentrations that blocked HSV-2 infection, with a CC₅₀ value of 282.1 ± 27.5 µM. The mechanism studies demonstrated that proanthocyanidin A-1 did not reduce viral infectivity but inhibited viral attachment and penetration and affected the late stage(s) of HSV-2 infection. It was concluded that proanthocyanidin A-1 suppressed HSV-2 infection through many modes of action and thus merits further investigation. Copyright © 2004 Society of Chemical Industry

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越橘原花青素A-1的体外抗HSV-2活性及其作用机制

本研究的目的是研究从越橘科植物越橘中分离得到的原花青素A-1的体外抗HSV-2活性及其作用机制。结果表明，原花青素A-1具有抗HSV-2的活性。XTT测定法的IC50值为73.3±14.5µM，斑块减少测定法的IC 50和IC90值分别为41.9±2.0和62.8±6.3µM。原花青素A-1在阻断HSV-2感染的浓度下没有显示出细胞毒性作用，CC50值为282.1±27.5µM。机制研究表明，原花青素A-1不会降低病毒感染性，但会抑制病毒附着和渗透，并影响HSV-2感染的晚期。结果表明，原花青素A-1通过多种作用方式抑制HSV-2感染，值得进一步研究。版权所有©2004化学工业协会

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.