Detection of insecticide detoxification enzymes activities in Aedes aegypti mosquito, the vector of dengue fever in Saudi Arabia

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2022-01-21 DOI:10.3233/mgc-210184
Abdullah G. Algamdi, J. Mahyoub
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引用次数: 4

Abstract

In this study, the sensitivity of Aedes aegypti mosquitoe larvae to four types of pesticides two phosphorous-based and two pyrethroid insecticides was investigated. The larvae revealed approximately 1.126-fold more susceptibility to Safrotin (LC50 = 0.127 ppm) than Fendure (LC50 = 0.143) in the case of phosphorous-based pesticides and approximately 1.265-fold more susceptibility to AlphaQuest (LC50 = 0.0347 ppm) than Klash (LC50 = 0.0439) in the case of pyrothroid insecticides. Comparison between groups revealed that AlphaQuest was more effective than Klash, Safrotin and Fendure by approximately 1.3, 3.6 and 4.1-fold, respectively. The presence of enzyme activities relevant to insecticide resistance was often evaluated using enzyme assays to detect the underlying resistance mechanisms that may not be detected using bioassays. In this study, the results revealed significant decrease in the activity of esterase, glutathione- S- transferase and acetylcholine esterase enzymes to varying degrees. There were significant increases in catalase and total glutathione activity in all treatments compared to the control. The study concluded that this variation in enzyme activities is due to the type and purity of the additive compound and the source and purity of the active ingredient. The study recommends using the catalase enzyme and total glutathione as a biomarker indication for pesticide synergistic intoxication in aquatic animals.
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沙特阿拉伯登革热病媒埃及伊蚊杀虫剂解毒酶活性检测
本研究研究了埃及伊蚊幼虫对4种杀虫剂(含磷杀虫剂和拟除虫菊酯类杀虫剂)的敏感性。对磷基杀虫剂,幼虫对Safrotin (LC50 = 0.127 ppm)的敏感性约为Fendure (LC50 = 0.143)的1.126倍,对AlphaQuest (LC50 = 0.0347 ppm)的敏感性约为Klash (LC50 = 0.0439)的1.265倍。组间比较显示,AlphaQuest比Klash、Safrotin和Fendure分别高出约1.3倍、3.6倍和4.1倍。通常使用酶分析来评估与杀虫剂抗性相关的酶活性的存在,以检测生物分析可能无法检测到的潜在抗性机制。本研究结果显示,酯酶、谷胱甘肽- S-转移酶和乙酰胆碱酯酶活性均有不同程度的显著降低。与对照组相比,所有处理的过氧化氢酶和总谷胱甘肽活性都有显著增加。研究得出结论,这种酶活性的变化是由于添加剂化合物的类型和纯度以及活性成分的来源和纯度。该研究建议使用过氧化氢酶和总谷胱甘肽作为水生动物农药协同中毒的生物标志物。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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