MALDI-TOF MS: Applications in Dairy and Related Sectors

Kamal Gandhi, Ashish Kumar, Prabin Sarkar, Ashok D.Aghav, D. Lal
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引用次数: 3

Abstract

MALDI-TOF MS is a soft ionization technique suitable for analysis of peptides, proteins, glycoproteins, oligosaccharides and oligonucleotides etc. In dairy sector, it can be used to analyze milk proteins, to identify adulteration in milk, isolation of phospholipids, characterization of proteins and enzymes of the microorganisms used in manufacture of cheese and other fermented products. In food industry, it can be used for evaluation and determination of fructo-oligosaccharides, inulins, certain pigments and flavor compounds present in different foods. This technique has found applications in a variety of disciplines including proteomics, glycomics, dairy, food, chemistry, biochemistry, microbiology, and physics. The purpose of this review is to summarize the contribution of the studies already performed with MALDI-TOF MS concerning dairy and related sectors. Keywords: MALDI-TOF, detector, mass analyser, laser, reflectron, spectrum, ionisation
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MALDI-TOF质谱:在乳制品及相关行业的应用
MALDI-TOF质谱是一种适用于多肽、蛋白质、糖蛋白、寡糖、寡核苷酸等分析的软电离技术。在乳制品行业,它可用于分析牛奶蛋白,识别牛奶中的掺假,分离磷脂,表征用于制造奶酪和其他发酵产品的微生物的蛋白质和酶。在食品工业中,可用于评价和测定不同食品中存在的低聚果糖、菊粉、某些色素和风味化合物。这项技术已经在多种学科中得到应用,包括蛋白质组学、糖组学、乳制品、食品、化学、生物化学、微生物学和物理学。本综述的目的是总结MALDI-TOF质谱法在乳制品及相关行业的研究成果。关键词:MALDI-TOF,检测器,质谱分析仪,激光,反射,光谱,电离
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