Effect of K-ras antisense oligodeoxynucleotides on human pancreatic cancer cell line PaTu 8988s

Abstract

OBJECTIVE: To study the effect of K-ras antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu 8988s at different times after treatment. METHODS: Human pancreatic cancer cells (PaTu 8988s) in exponential growth stage were used at a cell concentration of 1 × 105/mL; 0.5 mL of the cell suspension was placed in each well of replicate 24-well culture plates in the presence of different concentrations (50 and 100 μg/mL) of ASODN and sense oligodeoxynucleotides (SODN). Cell counts and 3-[4,5-dimethylthiazolzyl]-2,5-diphenyl tetrazolium bromide (MTT) assays were carried out 24, 48 and 72 h after treatment. RESULTS: At 12, 24, 48 and 72 h after ASODN treatment, the following rates of inhibition were observed: for 50 μg/mL, 42.3, 66.6, 69.6 and 74.6%, respectively; for 100 μg/mL, 66.2, 91.4, 98.2 and 98.3%, respectively. CONCLUSION: The inhibitory effect of ASODN began at 12 h post-treatment and became more marked at 48–72 h. The higher the concentration of ASODN, the earlier the peak of inhibitory rate appears.