Isolation and Identification of Escherichia albertii in Broiler Chickens From Kermanshah

Adel Akbari, Mostafa Razmjoo, A. Foroughi
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Abstract

Background: F Escherichia albertii is generally recognized as a human pathogen with a limited number of strains. It has also been identified as a cause of mortality among birds. The clinical significance and prevalence of E. albertii are somewhat unknown. Objectives: The aim of the present study was to isolate and identify E. albertii as a causative agent of respiratory infections in broilers. Materials and Methods: During a three-month period (Winter 2018), 200 samples of the air sacs of 100 chickens with suspected colibacillosis were collected. Routine biochemical tests were performed and suspected isolates of E. albertii were selected for polymerase chain reaction (PCR). Results: A total of 68 suspected samples of E. albertii and Escherichia coli were isolated. Further, E. coli was detected in all the suspected samples using species-specific sequences of E. coli (uidA) and E. albertii (mdh and lysP). Conclusion: Escherichia albertii was not identified as a cause of respiratory infection in broilers.
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克尔曼沙肉鸡阿尔伯氏杆菌的分离与鉴定
背景:阿尔伯氏埃希氏菌是一种公认的人类病原体,菌株数量有限。它也被确定为鸟类死亡的一个原因。阿尔伯蒂大肠杆菌的临床意义和流行程度尚不清楚。目的:本研究的目的是分离和鉴定阿尔伯氏杆菌是肉鸡呼吸道感染的病原体。材料与方法:在2018年冬季为期3个月的时间里,收集了100只疑似大肠杆菌病鸡的200个气囊样本。进行常规生化试验,选取疑似阿尔伯氏杆菌分离株进行聚合酶链反应(PCR)检测。结果:共分离出疑似阿尔伯蒂埃希菌和大肠埃希菌68份。此外,利用大肠杆菌(uidA)和阿尔伯蒂大肠杆菌(mdh和lysP)的种特异性序列在所有可疑样品中检测到大肠杆菌。结论:阿尔伯蒂埃希菌未被确定为肉鸡呼吸道感染的原因。
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