PROLIFERATION AND DIFFERENTIATION OF OSTEOBLAST-LIKE CELLS ON β-TRICALCIUM PHOSPHATE DOPED WITH MANGANESE (II) IONS

Abstract

Manganese (II) ions were substituted for Ca ions at the Ca(4) and Ca(5) sites located in the A column of the β-tricalcium phosphate (β-TCP) crystal structure, and effects of additions of Mn ions on proliferation and differentiation of osteoblasts were examined using mouse MC3T3-E1 osteoblast-like cells. The X-ray diffraction patterns of β-TCP doped with manganese (II) ions (Mn-β-TCP) indicated the formation of β-TCP solid solution to 13.64 mol%. MC3T3-E1 cells attached on Mn-β-TCP well proliferated compare to those on β-TCP. In particular, Mn-β-TCP doped with 3 mol% showed the most excellent cell proliferation and the highest ALP activity level. We consider that the new bone formation may be promoted by dissolution of calcium ions and manganese ions from Mn--TCP. The present Mn-β-TCP is expected as an implant biomaterial with enhanced bioactivity. (Received May 31, 2012; Accepted June 12, 2012)