{"title":"The immunomodulatory effects of bone marrow-derived mesenchymal stem cells on lymphocyte in spleens of aging rats","authors":"Zhi-Hong Wang, Zhi-Feng Lin, Yi-Ting Lai, Ling Ding, Huaibin Wang, Xin-gui Chen, Xiao-Ye Chen, Hua-Ke Zeng","doi":"10.53388/bmec2023013","DOIUrl":null,"url":null,"abstract":"Objective: To investigate the effects of bone marrow-derived mesenchymal stem cells (BMSCs) on the proliferation and secretion of IgM, IgG and IL-2 in spleen lymphocytes (L) of aging rats. Methods: BMSCs were isolated by the whole bone marrow adherence method and characterized. A rat model of aging was produced by daily subcutaneous injection of D-galactose into the back of the neck. Rat spleen lymphocyte isolate kit to isolate spleen lymphocytes from aging rats and young rats. In vitro , the co-culture system of BMSCs and aging rats lymphocytes was established, and under the induction of mitogen LPS and ConA, the proliferative activity of lymphocytes in each group was detected by CCK-8 assay, the levels of IgM and IgG in the culture supernatant of each group was detected by ELISA, and the IL-2 radioimmunoassay kits were used to detect the content of IL-2 in the supernatant of each group. Results: (1) The isolated adherent cells showed the characteristics of BMSCs, including spindle-shaped morphology, high expression of CD29, CD44, low expression of CD34 and CD45, and osteogenic/adipogenic ability. (2) Under LPS induction, lymphocyte proliferative activity and secretion of immunoglobulin IgG were reduced in the aging group compared with the young group, and co-culture with BMSCs reversed this trend. (3) Under ConA induction, the IL-2 content of BMSCs co-cultured with aging lymphocytes was higher than that of aging lymphocytes alone ( P < 0.0001); the IL-2 content of CsA co-cultured with aging lymphocytes was lower than that of aging lymphocytes alone ( P < 0.0001). Conclusion: BMSCs have immunomodulatory effects on the spleen lymphocytes of aging rats in vitro .","PeriodicalId":8862,"journal":{"name":"Biomedical Engineering: Applications, Basis and Communications","volume":"181 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Engineering: Applications, Basis and Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.53388/bmec2023013","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 1
Abstract
Objective: To investigate the effects of bone marrow-derived mesenchymal stem cells (BMSCs) on the proliferation and secretion of IgM, IgG and IL-2 in spleen lymphocytes (L) of aging rats. Methods: BMSCs were isolated by the whole bone marrow adherence method and characterized. A rat model of aging was produced by daily subcutaneous injection of D-galactose into the back of the neck. Rat spleen lymphocyte isolate kit to isolate spleen lymphocytes from aging rats and young rats. In vitro , the co-culture system of BMSCs and aging rats lymphocytes was established, and under the induction of mitogen LPS and ConA, the proliferative activity of lymphocytes in each group was detected by CCK-8 assay, the levels of IgM and IgG in the culture supernatant of each group was detected by ELISA, and the IL-2 radioimmunoassay kits were used to detect the content of IL-2 in the supernatant of each group. Results: (1) The isolated adherent cells showed the characteristics of BMSCs, including spindle-shaped morphology, high expression of CD29, CD44, low expression of CD34 and CD45, and osteogenic/adipogenic ability. (2) Under LPS induction, lymphocyte proliferative activity and secretion of immunoglobulin IgG were reduced in the aging group compared with the young group, and co-culture with BMSCs reversed this trend. (3) Under ConA induction, the IL-2 content of BMSCs co-cultured with aging lymphocytes was higher than that of aging lymphocytes alone ( P < 0.0001); the IL-2 content of CsA co-cultured with aging lymphocytes was lower than that of aging lymphocytes alone ( P < 0.0001). Conclusion: BMSCs have immunomodulatory effects on the spleen lymphocytes of aging rats in vitro .
期刊介绍：
Biomedical Engineering: Applications, Basis and Communications is an international, interdisciplinary journal aiming at publishing up-to-date contributions on original clinical and basic research in the biomedical engineering. Research of biomedical engineering has grown tremendously in the past few decades. Meanwhile, several outstanding journals in the field have emerged, with different emphases and objectives. We hope this journal will serve as a new forum for both scientists and clinicians to share their ideas and the results of their studies.
Biomedical Engineering: Applications, Basis and Communications explores all facets of biomedical engineering, with emphasis on both the clinical and scientific aspects of the study. It covers the fields of bioelectronics, biomaterials, biomechanics, bioinformatics, nano-biological sciences and clinical engineering. The journal fulfils this aim by publishing regular research / clinical articles, short communications, technical notes and review papers. Papers from both basic research and clinical investigations will be considered.
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