Extraction and characterization of antimicrobial pigment from marine bacteria and testing of antimicrobial activity against penicillin resistant

Abstract

The current study shows the extraction of pigment from pigment producing bacteria isolated from marine sediment. The isolation of pigment producing bacteria was done by spread plate method. The marine nutrient medium was used for the isolation procedure. Further, subculture was followed to get desired pure culture. From the isolated culture, various preliminary test, biochemical test, molecular characterization and antimicrobial activity were performed. To extract the pigment a solvent centrifugation process was carried out. The extracted pigment was tested against drug resistant Staphylococcus aureus. Based on the FTIR spectrum respective functional group were identified from extracted pigment. After GC-MS spectral analysis specific Neoisolangifolene (32.73%) compound was identified from the total pigment extract. UV-Visible spectral studies of the pigment extract have confirmed with the variations in absorption peak of total pigments. The isolated organisms were identified based on 16s rRNA partial gene sequence analysis identified as Micrococcus flavus.