Comparison of Rhodanese Activity and Distribution in Tomato (Solanum lycopersicum Mill.) Plant Parts and its Physicochemical Characterization

Abstract

Tomato (Solanum lycopersicum Mill.) which belongs to the family Solanaceae is one of the most important vegetable grown and consumed worldwide. Rhodanese activity distribution in the stem, leaf, green unripe fruit, yellow ripening fruit, and red ripe fruit tomato plant parts of tomato plant were compared. The yellow ripening fruit had the highest activity followed by the leaf then the stem then green unripe fruit, while the least activity was shown by the red ripe fruit. The activity difference between the red ripe fruit and those of the stem, leaf and yellow ripening fruit was statistically significant. Also, the activity difference between yellow ripening fruit and red ripe fruit was statistically significant. The purified rhodanese from the almond nuts had a specific activity of 4.45 RU/mg with yield of 0.2%. A Km value of 46.34 mM with Vmax 2.10 RU/ml/min were obtained from KCN while a Km value of 26.34 mM with Vmax of 1.52 RU/ml/min was obtained from N