Immobilization of Selected Microbes at Some Selected Solid Supports for Enhanced Fermentation Process

R. Hewawasam, C. Udawatte, S. Weliwegamage, S. Sotheeswaran, S. Rajapakse
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引用次数: 4

Abstract

Immobilization of macro molecules (such as enzymes) and micro-organisms can be generally defined as a procedure leading to their restricted mobility. Advantages of immobilization include easy separation of the enzymes/cells from the product and reuse of the enzymes/cells. In this research coconut tree leaf sheath was used to immobilize selected microbes which were used in fermentation tecnology. Coconut tree leaf sheath contains cellulose fiber layers which have cross linking between them. Sacchromyces cerevisiae was used as the microbial type due to widespread use in fermentation process. Microbes were entrapped within cellulose layers. Coconut tree leaf sheath was found to be an effecient solid support for immobilization. Immobilized microbes can be reused for fresh fermentation media. Immobilization can be carried out utilizing naturally avavilable coconut tree leaf sheath as a solid support, it`s usage is very cost-effective and eco-froiendly method rather than using synthetic or semi synthetic solid supports.
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特定微生物在特定固体载体上的固定化强化发酵过程
大分子(如酶)和微生物的固定化通常可以定义为导致其受限移动的过程。固定化的优点包括酶/细胞易于从产物中分离和酶/细胞的再利用。本研究以椰子树叶鞘为载体,对发酵工艺中选用的微生物进行固定化。椰子树叶鞘含有纤维素纤维层,它们之间有交联。由于酿酒酵母在发酵过程中被广泛使用,因此被用作微生物类型。微生物被包裹在纤维素层中。椰子树叶鞘是一种有效的固定固支物。固定化微生物可重复用于新鲜发酵培养基。利用天然可用的椰子树叶鞘作为固体支架进行固定,与使用合成或半合成固体支架相比,它的使用是一种非常具有成本效益和环保的方法。
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