MOLECULAR ANALYSIS OF A PUTATIVE EN/SPM-RELATED TRANSPOSON PROTEIN IN BRASSICA JUNCEA

A. Yassein, G. Eid, Nada F Hemeda, Zaki El Feky
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Abstract

Open reading frame (ORF) of Putative En/Spm transposon in Brassica potentially helps to understand the relationship between various eukaryotic transposable elements. The current study was initially conducted to isolate and analyze the putative En/Spm-related transposon gene from Brassica juncea. PCR products (750 bp) from B. juncea (accession PI 649105 and PI 271442) were cloned, sequenced, and analyzed. Results of BLAST showed identical sequences between two accessions with 100% similarity. The amplified DNA transposon and conserved domain compared to the GenBank database to evaluate the genetic diversity relationships. Sequence of this putative transposon gene from B. juncae was a 98% similar to B. rapa subsp pekinensis at the nucleotide level, and 94% with the En/Spm-related transposon protein of B. oleracea at the amino acid level. Conserved domain architecture was related to transposase_21_pfam0299 and transposase family tnp2 and had a relationship with space outside the cell structure and/or to space outside the plasma membrane.
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芥菜en / spm相关转座子蛋白的分子分析
芸苔属En/Spm转座子的开放阅读框(ORF)可能有助于理解真核生物中各种转座子之间的关系。本研究旨在分离和分析芥菜中与En/ spm相关的转座子基因。对芥菜B. juncea(编号PI 649105和PI 271442)的PCR产物(750bp)进行克隆、测序和分析。BLAST结果显示,两份材料的序列完全相同,相似度为100%。将扩增的DNA转座子和保守结构域与GenBank数据库进行比较,评估遗传多样性关系。该转座子基因序列在核苷酸水平上与B. rapa subsp pekinensis相似98%,在氨基酸水平上与B. toleracea En/ spm相关转座子蛋白相似94%。保守结构域结构与转座酶e_21_pfam0299和转座酶家族tnp2有关,并与细胞结构外空间和/或质膜外空间有关。
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