Screening of Botrytis Grey Mould Disease of Chickpea As Compared With Field Screening Techniques and Cut-Twig Method

Abstract

A field trial was carried out on the suppression of botrytis gray mould (BGM) of chickpea using various chickpea lines at Regional Agricultural Research Station (RARS), BARI, Rahmatpur, Barisal, Bangladesh during 2012-2013. Both field screening techniques and cut-twig method were applied to control the disease. Spore suspensions of BGM were applied at flower initiation stage in the field trial. In the field screening, out of 29 chickpea lines, the entries 14, 4, 9 and 2 showed 4, 5, 6 and 7 disease scoring scale, respectively. In case of cut-twig method out of 29 chickpea lines, the entries 4, 5, 10, 6 showed 4 5, 6, and 7 scoring scales, respectively. For both field screening and cut-twig method a total of four lines viz., ICCL 87322, ICC 4971, ICC 4936 and ICC 1680 entries showed resistant reaction to BGM. The highest yield under field screening technique was obtained from the line ICC 14559 (2403 kg/ha) followed by ICCX 860029-BH-1PNBPN-B (2161 kg/ha) and both are showed 4 grading scale. Although, field screening of chickpea genotypes is the widely practiced method, but screening in growth rooms and screen houses require temperature and humidity control facilities, that are not always available for researches in developing countries. Moreover, cut twig method is a quick and comprehensive screening technique and applicable to all areas in the world for controlling chickpea BGM. Keyword: Screening, BGM. Chickpea