{"title":"Microbiological Assay of Essential Amino Acids in Vegetables","authors":"Etsuko Fukuzumi","doi":"10.4327/JSNFS1949.18.69","DOIUrl":null,"url":null,"abstract":"18種類の葉菜類に直接塩酸またはアルカリを加え, 120℃, 15lb加熱水解して8種類の必須アミノ酸を微生物定量法により求めた。水解条件検討の結果Valine, Isoleucine, Tryptophanを除く他の5種のアミノ酸については, 2N-HCl 7時間加熱水解が適当であり, 各必須アミノ酸の再検率はキャベツのPhenylalanineを除いて他はいずれも良好であった。これら3種のアミノ酸測定のための適当な水解条件については, さらに検討の要があるが, 一応7時間水解試料について測定した値を文献値と比較した。分析結果を植物性蛋白質のアミノ酸と比較すると, 緑葉野菜のLysine/N, Methionine/N含量が一般に高く, 動物性蛋白質のそれらに近似の値を示すことを認めた。","PeriodicalId":86661,"journal":{"name":"[Eiyo to shokuryo] Journal of the Japanese Society of Food and Nutrition","volume":"20 1","pages":"69-72"},"PeriodicalIF":0.0000,"publicationDate":"1965-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"[Eiyo to shokuryo] Journal of the Japanese Society of Food and Nutrition","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4327/JSNFS1949.18.69","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}