Chemical composition and selective BuChE activity of the essential oil of Piper arboreum Aubl. from Ecuador

IF 0.7 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2023-09-01 DOI:10.37360/blacpma.23.22.5.45

Lisseth Carolina Espinosa Espinoza, Melissa Salinas Rivera, N. Bec, Jorge Ramirez Robles, C. Larroque, Chabaco Armijos

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Abstract

The aim of this study was to identify the chemical composition of the Piper arboreumAubl. essential oil (EO), and to evaluate its inhibitory activity in vitroagainst the enzymes butyrylcholinesterase (BuChE) and acetylcholinesterase (AChE). The EO was obtained by steam distillation of the leaves, which were collected in Pindal canton of the Loja province in southern Ecuador. The chemical composition was analyzed using the gas chromatography technique coupled to a mass spectrometry detector (GC-MS) and gas chromatography coupled to a flame ionization detector (GC-FID). A total of 41 compounds were identified, the major components found in the oil were limonene (31,46%), β-selinene (12,01%), (E)-caryophyllene (7,53%), bicyclogermacrene (6,72%), germacrene D (3,83%) and β-elemene (3,63%). In in vitro analyzes, the EO showed high selective inhibition for BuChE with an IC50inhibition value of 29,3±3,3 μg/mL. By contrast, the EO was not active against the AChE enzyme (IC50was 100,1±15,2 μg/mL).

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花椒精油的化学成分及选择性BuChE活性研究。从厄瓜多尔

本研究的目的是鉴定胡椒属植物的化学成分。精油(EO)，并评价其对丁酰胆碱酯酶(BuChE)和乙酰胆碱酯酶(AChE)的体外抑制活性。EO是通过蒸汽蒸馏法获得的，这些叶子是在厄瓜多尔南部洛哈省的平达尔州收集的。化学成分分析采用气相色谱联用质谱检测器(GC-MS)和气相色谱联用火焰离子化检测器(GC-FID)。共鉴定出41种化合物，主要成分为柠檬烯(31,46%)、β-亚麻烯(12,01%)、(E)-石竹烯(7,53%)、双环槐烯(6,72%)、槐烯D(3,83%)和β-榄香烯(3,63%)。体外分析表明，EO对BuChE具有高选择性抑制作用，ic50抑制值为29.3±3.3 μg/mL。而EO对AChE无抑制作用(ic50分别为100、1±15.2 μg/mL)。

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.