Pengaruh Hormon terhadap Induksi Embrio Somatik Kacapiring (Gardenia jasminoides) dan Potensi Aplikasinya dalam Pembuatan Benih Sintetik

Yosua Pardede, E. Mursyanti, B. R. Sidharta
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Abstract

Kacapiring (Gardenia jasminoides) is an ornamental plant that has many uses, as a cut flowers, food ingredients, textile materials, cosmetic raw materials, and containing therapeutic compounds. The high demand for gardenia needs to be balanced with the results of its cultivation. Conventional propagation of gardenias has disadvantages such as a long life cycle, genotypes can differ from the parent, and can carry disease from the parent. Therefore, more efficient gardenia propagation techniques are needed, one of which is by making synthetic seeds. Synthetic seeds can be produced by encapsulating somatic embryos. Somatic embryos are collections of embryonic cells formed from soma cells that have the ability to regenerate into a complete new plant. Hormones are organic materials that occur naturally in plants or are synthetic. Hormones have an effect on plant differentiation and growth. Somatic embryogenesis can be carried out using auxin and cytokinin hormones, because auxins can induce embryogenic callus and cytokinins can initiate the embryogenic process in callus. Synthetic seeds are single somatic embryos covered by a matrix, so that they can be stored for a certain duration of time and can grow into a complete plant in both in vitro and ex vitro conditions. This literature review is to provide an overview based on existing references on the effects of various types of hormones for the induction of gardenia somatic embryos, synthetic seed production methods, and synthetic seed storage temperatures.
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荷尔蒙对身体胚胎的诱导成型牙髓的影响,以及合成种子的潜在应用
栀子(栀子花)是一种具有多种用途的观赏植物,可作为切花、食品原料、纺织材料、化妆品原料和含有治疗化合物。对栀子花的高需求需要与栽培结果相平衡。栀子传统繁殖存在生命周期长、基因型与亲本不同、携带疾病等缺点。因此,需要更有效的栀子花繁殖技术,其中之一就是合成种子。合成种子可以通过包裹体细胞胚胎产生。体细胞胚胎是由体细胞形成的胚胎细胞的集合,具有再生成一个完整的新植物的能力。激素是天然存在于植物体内或人工合成的有机物质。激素对植物的分化和生长有影响。生长素和细胞分裂素可以诱导体细胞胚胎发生,因为生长素可以诱导胚胎发生愈伤组织,细胞分裂素可以启动愈伤组织的胚胎发生过程。合成种子是由基质覆盖的单个体细胞胚胎,因此它们可以保存一定的时间,并且在离体和离体条件下都可以长成完整的植物。本文在现有文献的基础上,对各种激素对栀子体细胞胚诱导的影响、合成制种方法、合成种子贮藏温度等方面进行综述。
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