Effects of co-treatment of Rauwolfia vomitoria and Gongronema latifolium on neurobehaviour and the neurohistology of the cerebral cortex in mice

Moses B. Ekong, U. Ekpene, F. Thompson, A. Peter, Nab Udoh, G. J. Ekandem
{"title":"Effects of co-treatment of Rauwolfia vomitoria and Gongronema latifolium on neurobehaviour and the neurohistology of the cerebral cortex in mice","authors":"Moses B. Ekong, U. Ekpene, F. Thompson, A. Peter, Nab Udoh, G. J. Ekandem","doi":"10.4314/IJMU.V10I1.2","DOIUrl":null,"url":null,"abstract":"Rauwolfia vomitoria and Gongronema latifolium are medicinal plants with antioxidant, antidiabetic and analgesic properties among others. R. vomitoria is reported to possess adverse neural effects, which G. latifolium has shown the potential to address. This study therefore investigated the effects of co-treatment of R. vomitoria and G. latifolium on the neurobehaviour and histology of the cerebral cortex of female mice. Twenty female Wistar mice were divided into 4 groups (A, B, C and D). Group A designated as the control received 0.4 mL of 20 % Tween, while groups B, C and D received oral doses of 150 mg/kg of R. vomitoria (RV), 200 mg/kg of G. latifolium (GL) and a combination of 150 mg/kg of R. vomitoria and 200 mg/kg of G. latifolium (RV+GL), respectively for seven days. Light and dark field behaviour test was carried out on day 8 and the animals were immediately sacrificed. Their brains were excised and routinely processed by haematoxylin and eosin method. There was no difference in body and brain weights, and the behavioural parameters. Cellular cyto-architecture showed higher glial population with no apparent histopathology. The cellular population was higher (p<0.0001) in the RV and RV+GL groups, while the GL group was less (p<0.0001) populated all compared to the control.In conclusion, the reported treatment regimes, RV administered singly and in combination with GL may not affect some neurobehavioural activities, but may result in cellular increase in the cerebral cortex. KEY WORDS : Rauwolfia vomitoria; Gongronema latifolium ; Cerebral cortex; Mice","PeriodicalId":14472,"journal":{"name":"Internet Journal of Medical Update - EJOURNAL","volume":"5 1","pages":"3-10"},"PeriodicalIF":0.0000,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Internet Journal of Medical Update - EJOURNAL","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/IJMU.V10I1.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11

Abstract

Rauwolfia vomitoria and Gongronema latifolium are medicinal plants with antioxidant, antidiabetic and analgesic properties among others. R. vomitoria is reported to possess adverse neural effects, which G. latifolium has shown the potential to address. This study therefore investigated the effects of co-treatment of R. vomitoria and G. latifolium on the neurobehaviour and histology of the cerebral cortex of female mice. Twenty female Wistar mice were divided into 4 groups (A, B, C and D). Group A designated as the control received 0.4 mL of 20 % Tween, while groups B, C and D received oral doses of 150 mg/kg of R. vomitoria (RV), 200 mg/kg of G. latifolium (GL) and a combination of 150 mg/kg of R. vomitoria and 200 mg/kg of G. latifolium (RV+GL), respectively for seven days. Light and dark field behaviour test was carried out on day 8 and the animals were immediately sacrificed. Their brains were excised and routinely processed by haematoxylin and eosin method. There was no difference in body and brain weights, and the behavioural parameters. Cellular cyto-architecture showed higher glial population with no apparent histopathology. The cellular population was higher (p<0.0001) in the RV and RV+GL groups, while the GL group was less (p<0.0001) populated all compared to the control.In conclusion, the reported treatment regimes, RV administered singly and in combination with GL may not affect some neurobehavioural activities, but may result in cellular increase in the cerebral cortex. KEY WORDS : Rauwolfia vomitoria; Gongronema latifolium ; Cerebral cortex; Mice
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
联合治疗呕吐虫和松茸对小鼠神经行为和大脑皮层神经组织学的影响
牛油果和大黄是具有抗氧化、抗糖尿病和镇痛作用的药用植物。据报道,R. vomitoria具有不良的神经效应,这是G. latifolium已经显示出解决的潜力。因此,本研究探讨了呕吐草和阔叶草共同治疗对雌性小鼠大脑皮层神经行为和组织学的影响。雌性Wistar小鼠20只,分为A、B、C、D 4组,A组作为对照组,给予0.4 mL 20%的吐温,B、C、D组分别口服150 mg/kg呕吐物(RV)、200 mg/kg latifolium (GL)以及150 mg/kg呕吐物和200 mg/kg latifolium (RV+GL),连续7 D。第8天进行明暗场行为试验,立即处死。他们的大脑被切除,并用血红素和伊红法进行常规处理。在身体和大脑重量以及行为参数方面没有差异。细胞结构显示较高的胶质细胞群,未见明显的组织病理学变化。RV组和RV+GL组细胞数量均高于对照组(p<0.0001),而GL组细胞数量均低于对照组(p<0.0001)。总之,报告的治疗方案,RV单独和联合GL可能不会影响某些神经行为活动,但可能导致大脑皮层细胞增加。毕业论文关键词:水蚤;龙葵;大脑皮层;老鼠
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Editorial: An overview of gamblers anonymous Herpes encephalitis in an elderly immunocompetent lady – A case report Traumatic long bone fractures in children seen in a metropolitan tertiary hospital in Enugu, Nigeria Laparoscopic colorectal cancer surgery - a prospective study of short-term outcomes of consecutive cases over 3 years Editorial : Plagiarism
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1