Local Ionic Environment of Plant Membranes: Effects on Membrane Functions

Jean-Baptiste Thibaud , Charles Romieu , Remy Gibrat , Jean-Pierre Grouzis , Claude Grignon
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引用次数: 21

Abstract

Electrostatic properties of the isolated plasma membrane of maize roots were studied using the fluorescent ANS probe and microelectrophoresis of membrane vesicles. Variations of the net surface charge of membrane were obtained by adding ionic surfactants or by acidifying the medium. Parallel experiments were performed on excised roots. We showed that surface electrostatic interactions can affect major membrane functions such as ATPase activity, trans-membrane PD, and permeability to NO3.

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植物膜局部离子环境对膜功能的影响
采用荧光ANS探针和膜泡微电泳技术研究了玉米根质膜的静电特性。通过添加离子表面活性剂或酸化介质,获得了膜表面净电荷的变化。对切除的根进行平行实验。我们发现,表面静电相互作用可以影响主要的膜功能,如atp酶活性、跨膜PD和对NO3的渗透性。
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