The role of IL-13 Polymorphisms in Asthma

Abstract

Background: Asthma is a chronic respiratory disease with variation in induces clinical symptoms and response to therapy. Several IL-13 single nucleotide polymorphisms (SNPs) associated with asthma predisposing in particular populations. IL-13 variants may play role in dysregulation of immunoglobulin E. Aims to the study association of single nucleotide polymorphisms (SNPs)of IL13 gene (rs20541 and rs1295686) with asthma. Methods and materials: Subjects and methods total of ninety (90) adult patients with asthma were included in the current study. 51 (56.7%) were females and 39 (43.3%) were male. Their age range from 18 to 75 years compared to ninety (90) of apparently healthy people as control group. The detection of IL-13 serum levels by using ELISA, while the determination of IL-13 Gene polymorphisms (rs20541 and rs1295686) by using Conventional-PCR (Polymerase Chain Reaction). Results: this study revealed that the serum level of IL-13 was significantly differences between asthmatic and control groups (pvalue =0.02). Regarding IL-13 rs20541, the results revealed a statistically significant difference between patients and control for genotypes GA, AA (Pvalue = 0.0167, Pvalue = 0.021 respectively) and allele frequency (A) p-value 0.0002, on other hand the IL-13 rs1295686 revealed statistically significant difference between patients and control for genotypes CT, TT (P-value = 0.0009, P-value = 0.0005) respectively and allele frequency (T) with p-value <0.0001.Conclusion: In conclusion, the increased frequency in genotypes (GA, AA and CT, TT) of IL-13 polymorphisms (rs20541 and rs1295686) respectively, and “A”,” T” alleles of these variants could be considered as a risk factors for asthma developing.