MiR-708-5p as a Predictive Marker of Colorectal Cancer Prognosis

P. F. Fedatto, Thais Inácio de Carvalho, J. C. Oliveira, D. Antônio, J. Pezuk, D. Tirapelli, O. Féres, J. J. Rocha, C. Scrideli, L. Tone, M. Brassesco
{"title":"MiR-708-5p as a Predictive Marker of Colorectal Cancer Prognosis","authors":"P. F. Fedatto, Thais Inácio de Carvalho, J. C. Oliveira, D. Antônio, J. Pezuk, D. Tirapelli, O. Féres, J. J. Rocha, C. Scrideli, L. Tone, M. Brassesco","doi":"10.6000/1927-7229.2016.05.01.2","DOIUrl":null,"url":null,"abstract":"Background : MicroRNAs (miRNA) are short non-coding RNA that act as negative regulators of gene expression. Altered levels of miR-708-5p have recently been described in many tumors, although its contribution in colorectal cancer (CRC) pathophysiology remains unclear. Methods/Patients : Quantitative real-time polymerase chain reaction was employed to evaluate the expression of miR-708-5p in 50 CRC and 20 paired adjacent noncancerous tissues. The relationship between miRNA levels and clinicopathological features was estimated using the Mann-Whitney test, and survival curves calculated by the Kaplan-Meier method. Additionally, in vitro assays were performed to investigate the possible role of miR-708-5p on CRC cell survival. Results : The expression level of miR-708-5p was significantly decreased in CRC tissues (3.79 fold-change, p=0.0112) when compared with non-neoplastic colon samples. Paired analysis in 20 CRC samples with their corresponding adjacent non-neoplastic tissue showed miR-708 downregulation in 60% of them. The same pattern was seen in DLD1 and HT-29 cell lines (~50-fold decrease). Interestingly, higher expression is observed in patients with poor prognosis such as stage III/IV, relapse/metastasis and death, and shorter 5-year event free survival. Exogenous expression of miR-708 exerted a significant influence on clonogenicity in vitro . Conclusion : These results suggest that reduced miR-708-5p expression may contribute to the first stages of colorectal carcinogenesis. A shift in the regulation of miR-708-5p might operate in more severe stages of the disease. It seems that lower levels of miR-708 expression might connote less advanced disease and better prognosis. Further studies are needed to corroborate our results and better elucidate the role of miR-708 in CRC.","PeriodicalId":14957,"journal":{"name":"Journal of Analytical Oncology","volume":"15 1","pages":"14-23"},"PeriodicalIF":0.0000,"publicationDate":"2016-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Analytical Oncology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.6000/1927-7229.2016.05.01.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

Background : MicroRNAs (miRNA) are short non-coding RNA that act as negative regulators of gene expression. Altered levels of miR-708-5p have recently been described in many tumors, although its contribution in colorectal cancer (CRC) pathophysiology remains unclear. Methods/Patients : Quantitative real-time polymerase chain reaction was employed to evaluate the expression of miR-708-5p in 50 CRC and 20 paired adjacent noncancerous tissues. The relationship between miRNA levels and clinicopathological features was estimated using the Mann-Whitney test, and survival curves calculated by the Kaplan-Meier method. Additionally, in vitro assays were performed to investigate the possible role of miR-708-5p on CRC cell survival. Results : The expression level of miR-708-5p was significantly decreased in CRC tissues (3.79 fold-change, p=0.0112) when compared with non-neoplastic colon samples. Paired analysis in 20 CRC samples with their corresponding adjacent non-neoplastic tissue showed miR-708 downregulation in 60% of them. The same pattern was seen in DLD1 and HT-29 cell lines (~50-fold decrease). Interestingly, higher expression is observed in patients with poor prognosis such as stage III/IV, relapse/metastasis and death, and shorter 5-year event free survival. Exogenous expression of miR-708 exerted a significant influence on clonogenicity in vitro . Conclusion : These results suggest that reduced miR-708-5p expression may contribute to the first stages of colorectal carcinogenesis. A shift in the regulation of miR-708-5p might operate in more severe stages of the disease. It seems that lower levels of miR-708 expression might connote less advanced disease and better prognosis. Further studies are needed to corroborate our results and better elucidate the role of miR-708 in CRC.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
MiR-708-5p作为结直肠癌预后的预测标志物
背景:MicroRNAs (miRNA)是一种短的非编码RNA,是基因表达的负调控因子。miR-708-5p水平的改变最近在许多肿瘤中被描述,尽管其在结直肠癌(CRC)病理生理中的作用尚不清楚。方法/患者:采用实时定量聚合酶链反应(pcr)评估miR-708-5p在50例结直肠癌和20对癌旁非癌组织中的表达。使用Mann-Whitney检验估计miRNA水平与临床病理特征之间的关系,使用Kaplan-Meier法计算生存曲线。此外,我们还进行了体外实验来研究miR-708-5p对CRC细胞存活的可能作用。结果:结直肠癌组织中miR-708-5p的表达水平与非肿瘤结肠样本相比显著降低(3.79倍变化,p=0.0112)。对20个CRC样本及其相应的邻近非肿瘤组织的配对分析显示,其中60%的样本中miR-708下调。在DLD1和HT-29细胞系中也观察到相同的模式(约下降50倍)。有趣的是,在预后较差的患者(如III/IV期、复发/转移和死亡)中观察到较高的表达,并且5年无事件生存期较短。在体外,外源表达miR-708对克隆原性有显著影响。结论:这些结果表明miR-708-5p表达的降低可能与结直肠癌的第一阶段发生有关。miR-708-5p调控的转变可能在疾病的更严重阶段起作用。似乎较低水平的miR-708表达可能意味着更少的疾病进展和更好的预后。需要进一步的研究来证实我们的结果,并更好地阐明miR-708在CRC中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
New Insights on Hyperbaric Oxygen Therapy for Cancer Chemically Induced Brain Cancer in Sprague-Dawley Rats: Changed Lipidomics Mimics the Human Conditions Multiple Primary Malignant Tumours Magnetotherapy in Experimental and Clinical Neuro-Oncology: A Review Uterine Metastasis from Carcinoma of Breast – A Systematic Analysis
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1