A Novel Fluorescent Protein Pair for Dual-color Two-photon Laser Scanning Microscopy*: A Novel Fluorescent Protein Pair for Dual-color Two-photon Laser Scanning Microscopy*
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引用次数: 0
Abstract
Dual-color two-photon laser scanning microscopy is a useful method for simultaneously studying the expression, localization and trafficking of two different proteins in tissues. Because most two-photon microscopes only use a single wavelength excitation laser, simultaneously exciting multiple fluorescent proteins remains a challenge. Here, we present mAmetrine and mKate2, which can be used as a novel fluorescent protein pair in dual-color two-photon imaging by taking advantage of the large Stokes shift of mAmetrine and high brightness of mKate2. Both proteins have high two-photon absorption efficiencies and can be simultaneously excited at an optical wavelength of 765 nm. Dual-color two-photon imaging using this protein pair is highly effective in living cells.
期刊介绍：
Progress in Biochemistry and Biophysics is a monthly, peer-reviewed journal sponsored by Institute of Biophysics, The Chinese Academy of Sciences together with Biophysical Society of China. It started publication in 1974. It mainly reports on the latest developments in biochemistry, biophysics, molecular biology and neuroscience both in China and abroad. It contains several columns such as "reviews and monographs", "rapid and short communications", "research papers", "techniques and methods" and "academic discussions".
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