Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens

IF 0.6 4区 农林科学 Q3 VETERINARY SCIENCES Veterinarni Medicina Pub Date : 2021-05-31 DOI:10.17221/190/2020-VETMED
Kristina Putecova, K. Nedbalcová, I. Bartejsova, M. Zouharova, Katarína Matiašková, E. Jeklová, Michaela Viskova, Petra Zouzelkova, Martin Jeřábek, K. Šťastný
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引用次数: 3

Abstract

A rapid, simple and highly efficient analytical method for the targeted determination of trimethoprim and sulfamethoxazole in serum samples has been developed and used to measure the pharmacokinetic curve of these medicinal substances after administration to chicken broilers. The pharmacokinetics properties of trimethoprim and sulfamethoxazole were investigated in clinically healthy broiler chickens after the single oral administration of the commercial preparation Methoxasol (Eurovet Animal Health, B.V., The Netherlands) at a dose of 0.275 ml/kg b.w. After a single dose drug administration, the chickens were sacrificed by decapitation under general anaesthesia by Isoflurin 1 000 mg/g (Vetpharma AH, Spain) and the blood was collected at precisely defined intervals: 15, 30, 45, 60, 90, 120, 180, 360 and 720 min after the administration. The serum concentrations of amoxicillin were determined using Q Exactive tandem mass spectrometer (Thermo Fisher Scientific, USA) in conjunction with liquid chromatography. The detected pharmacokinetic parameters of trimethoprim after the oral administration were Cmax = 2.1 ± 1.0 μg/ml; Tmax = 1.5 h; t1⁄2 = 0.88 h; kel = 0.009 3 ± 0.001 1 1/h; AUCt = 2.901 ± 1.4 μg.h/ml; AUC∞ = 2.907 ± 1.5 μg.h/ml; Vd = 2.632 l/kg; Cl = 2.7 l/h. The pharmacokinetic parameters of sulfamethoxazole after the oral administration were Cmax = 47.1 ± 15.3 μg/ml; Tmax = 1 h; t1⁄2 = 1.92 h; kel = 0.004 6 ± 0.000 3 1/h; AUCt = 89.676 ± 26.9 μg.h/ml; AUC∞ = 94.612 ± 28.4 μg.h/ml; Vd = 0.584 l/kg; Cl = 0.21 l/h. To the best of our knowledge, this is the first pharmacokinetic study of the combination of sulfamethoxazole and trimethoprim in broiler chickens.
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甲氧苄啶与磺胺甲恶唑复方在肉鸡血清中的药动学特性的实验研究
建立了一种快速、简便、高效的测定血清样品中甲氧苄啶和磺胺甲恶唑含量的分析方法,并用于测定这两种药物给药后的药动学曲线。以0.275 ml/kg b.w的剂量单次口服甲氧苄氨嘧啶(Eurovet Animal Health, b.v., The Netherlands)商业制剂甲氧苄啶和磺胺甲恶唑在临床健康肉鸡体内的药代动力学特性进行了研究。单次给药后,用异氟灵1 000 mg/g (Vetpharma AH, Spain)全麻处死鸡,并按精确规定的时间间隔采集血液:给药后15、30、45、60、90、120、180、360、720分钟。血清阿莫西林浓度测定采用Q - Exactive串联质谱仪(Thermo Fisher Scientific, USA)结合液相色谱法。口服甲氧苄啶后检测的药动学参数为Cmax = 2.1±1.0 μg/ml;Tmax = 1.5 h;T1 / 2 = 0.88 h;Kel = 0.009 3±0.001 1 1/h;AUCt = 2.901±1.4 μg.h/ml;AUC∞= 2.907±1.5 μg.h/ml;Vd = 2.632 l/kg;Cl = 2.7 l/h。磺胺甲恶唑口服后药动学参数为Cmax = 47.1±15.3 μg/ml;Tmax = 1 h;T1 / 2 = 1.92 h;Kel = 0.004 6±0.000 3 1/h;AUCt = 89.676±26.9 μg.h/ml;AUC∞= 94.612±28.4 μg.h/ml;Vd = 0.584 l/kg;Cl = 0.21 l/h。据我们所知,这是首次对磺胺甲恶唑和甲氧苄啶在肉鸡体内的联合药代动力学研究。
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来源期刊
Veterinarni Medicina
Veterinarni Medicina Veterinary Sciences-兽医学
CiteScore
1.30
自引率
0.00%
发文量
62
审稿时长
18-36 weeks
期刊介绍: The journal Veterinarni Medicina publishes in English original papers, short communications, critical reviews and case reports from all fields of veterinary and biomedical sciences.
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