Antimicrobial susceptibility of coagulase-negative staphylococci isolated from red-tailed racers (Gonyosoma oxycephalum)

Lydia Pow Kar Men, U. Afiff, D. Noviana
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Abstract

Antibiotic resistant Coagulase negative staphylococci CoNS have been reported around the world. Therefore, the aim of the study is to determine the antimicrobial susceptibility of CoNS isolated from red–tailed racers. Samples were swabbed from the oral cavity of 5 wild caught red–tailed racers, and were identified with biochemical test. The results obtained show that red–tailed racer 1 had S. sciuri and S. xylosus; red–tailed racer 2 had S. xylosus; red–tailed racer 3 had S. sciuri; red–tailed racer 4 had S. lentus; and red–tailed racer 5 had S. kloosii.  The resistance test was done using the Kirby–Bauer disc diffusion test. And interpreted by referring to Clinical and Laboratory Standards Institute (CLS1 2013). The results for antibiotic resistance test of  Staphylococcus xylosus, Staphylococcus sciuri, and Staphylococcus lentus show susceptibility to amoxicillin, gentamicin, erythromycin, bacitracin, vancomycin and oxacillin, but resistance towards penicillin G. One sample of Staphylococcus sciuri is intermediate towards erythromycin, and one sample of Staphylococcus kloosii shows susceptibility towards amoxicillin, gentamicin, bacitracin, penicillin G, vancomycin, and oxacillin, but is resistant towards erythromycin. Keywords: antimicrobial susceptibility, coagulase negative staphylococci, red–tailed racers, Gonyosoma oxycephalum
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血凝酶阴性葡萄球菌的药敏分析
抗生素耐药凝固酶阴性葡萄球菌在世界各地均有报道。因此,本研究的目的是确定从红尾race中分离的CoNS的抗菌敏感性。从5只野生捕获的红尾鼠口腔中提取样本,进行生物化学鉴定。结果表明:红尾小蠊1号具有穗孢菌和木藻菌;红尾赛跑者2号有木藻;红尾赛车3号感染了猪链球菌;红尾赛车4号有lentus;红尾赛车5号感染了S. kloosii。阻力试验采用Kirby-Bauer圆盘扩散试验。并参照临床与实验室标准协会(CLS1 2013)解释。木葡萄球菌、人类葡萄球菌和香菇葡萄球菌耐药试验结果显示,对阿莫西林、庆大霉素、红霉素、杆菌霉素、万古霉素和恶西林敏感,但对青霉素G耐药,1份人类葡萄球菌对红霉素敏感,1份克氏葡萄球菌对阿莫西林、庆大霉素、杆菌霉素、青霉素G、万古霉素和恶西林敏感。但对红霉素有耐药性。关键词:抗菌素敏感性,凝固酶阴性葡萄球菌,红尾赛菌,氧头Gonyosoma
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