{"title":"Protective effect of progesterone on rat optic nerve after optic nerve crush","authors":"Hai-yan Sun, Wenting Sun, G. An","doi":"10.3760/CMA.J.ISSN.1006-4443.2017.08.022","DOIUrl":null,"url":null,"abstract":"Objective \nTo observe protective effect of progesterone on rat Optic Nerve after optic nerve crush. \n \n \nMethods \nFifty female SD rats were randomly divided into three groups, normal group with 10 rats (20 eyes, the normal group had no treatment), control group with 20 rats and treatment group with 20 rats. Mis-classification damage analogues were made on control group and treated group. For the treatment group, progesterone was injected to the rats’ abdominal cavity (10mg/kg) after one hour of injury, for once a day and until the end of experiment. For the control group, distilled water was injected to the rats’ abdominal cavity (10mg/kg) after one hour of injury, for once a day and until the end of experiment. Four optic retinas of every group were taken. Fixed, embedded, sliced for HE staining to observe the morphological changes of the retina and detect the mean OD value of IGF-1 and AQP-4. \n \n \nResults \nThe expression of IGF-1 in control group enhanced significantly after 1 day, 3 days and 7days of injury (P <0.05) and began to decrease at the 14th day.The expressions of IGF-1 in treatment group were significantly obvious than that of control group at every time point after injury and there was significant difference between the two group (P <0.05). The expression of AQP-4 in control group began to increase after one day of injury, reached the highest level at the third day, began to decrease at the 7th day and closed to normal level at the 28th day. Except for the 28th day the positive expressions of AQP-4 in treatment group at the first day, 3rd day, 7th day and 14th day were significantly lower than that of control. \n \n \nConclusions \nThe expression of IGF-1 and AQP-4 in normal retinal tissue is positive and it increases after incomplete injury of the optic nerve. IGF-1 and AQP-4 participate the process of incomplete injury of rats’ optic nerve. Progesterone can decrease the expression of AQP-4 and increase the expression of IGF-1 in the rat retinal tissue after the incomplete injury of optic nerve. Progesterone can promote the regeneration and repair of retina cell. \n \n \nKey words: \nOptic nerve; Progesterone; Retina ganglion cell; Insulin-like growth factor-1; Aquaporin-4","PeriodicalId":10236,"journal":{"name":"中国实用眼科杂志","volume":"31 1","pages":"836-840"},"PeriodicalIF":0.0000,"publicationDate":"2017-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国实用眼科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1006-4443.2017.08.022","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective
To observe protective effect of progesterone on rat Optic Nerve after optic nerve crush.
Methods
Fifty female SD rats were randomly divided into three groups, normal group with 10 rats (20 eyes, the normal group had no treatment), control group with 20 rats and treatment group with 20 rats. Mis-classification damage analogues were made on control group and treated group. For the treatment group, progesterone was injected to the rats’ abdominal cavity (10mg/kg) after one hour of injury, for once a day and until the end of experiment. For the control group, distilled water was injected to the rats’ abdominal cavity (10mg/kg) after one hour of injury, for once a day and until the end of experiment. Four optic retinas of every group were taken. Fixed, embedded, sliced for HE staining to observe the morphological changes of the retina and detect the mean OD value of IGF-1 and AQP-4.
Results
The expression of IGF-1 in control group enhanced significantly after 1 day, 3 days and 7days of injury (P <0.05) and began to decrease at the 14th day.The expressions of IGF-1 in treatment group were significantly obvious than that of control group at every time point after injury and there was significant difference between the two group (P <0.05). The expression of AQP-4 in control group began to increase after one day of injury, reached the highest level at the third day, began to decrease at the 7th day and closed to normal level at the 28th day. Except for the 28th day the positive expressions of AQP-4 in treatment group at the first day, 3rd day, 7th day and 14th day were significantly lower than that of control.
Conclusions
The expression of IGF-1 and AQP-4 in normal retinal tissue is positive and it increases after incomplete injury of the optic nerve. IGF-1 and AQP-4 participate the process of incomplete injury of rats’ optic nerve. Progesterone can decrease the expression of AQP-4 and increase the expression of IGF-1 in the rat retinal tissue after the incomplete injury of optic nerve. Progesterone can promote the regeneration and repair of retina cell.
Key words:
Optic nerve; Progesterone; Retina ganglion cell; Insulin-like growth factor-1; Aquaporin-4
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