Enzymatic saccharification of sodium hypochlorite treated bagasse and cotton stalks

Nadia Naim, Abdel-Mohsen S. Ismail, Lotfy A. Sallam, Abdel-Monem H. El-Refai
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引用次数: 2

Abstract

Attention was devoted to the delignification of the crude bagasse (CB) and cotton stalks (CS) by cheap commercial sodium hypochlorite solution.

Optimum delignification of these substrates was achieved by treatment with 0·7 g Cl g−1 substrate (pH 7·0) for 20 h at room temperature, with a solid: liquid (S:L) ratio 1:20.

The delignified CB and CS were saccharified with an enzyme preparation from a potent local mutant of Trichoderma viride (253-M16). A satisfactory saccharification was obtained with most of the treatments used. The highest conversion yield (93%) was recorded with CB treated with sodium hypochlorite solution (S:L ratio 1:20), followed by NaOH (0·05 m).

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次氯酸钠处理甘蔗渣和棉花秸秆的酶法糖化研究
研究了廉价的次氯酸钠溶液对粗甘蔗渣(CB)和棉花秸秆(CS)的脱木质素作用。在室温下,以0.7 g Cl g−1底物(pH 7.0),固液比1:20,处理20 h,达到最佳脱木质素效果。将去木素化的黑炭黑和黑炭黑用一种强力的绿色木霉(Trichoderma viride, 253-M16)本地突变体酶制剂进行糖化处理。多数处理均取得满意的糖化效果。次氯酸钠溶液(S:L: 1:20)处理CB的转化率最高(93%),NaOH溶液(0.05 m)次之。
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