Multiple locations of the rRNA genes in chromosomes of pines, Pinus densiflora and P. thunbergii

M. Hizume, F. Ishida, M. Murata
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引用次数: 27

Abstract

The ribosomal RNA (rRNA) genes were mapped on somatic chromosomes of Japanese red pine (Pinus densiflora) and Japanese black pine (P. thunbergii) by in situ hybridization using biotin-labeled 18S-25S rDNA. Cytochemical detection of the hybridized probe showed many signals in the interphase nuclei and, prophase and metaphase chromosomes in both species. In the metaphase complement of P. densiflora, the signals appeared singly at the interstitial region of seven pairs of the chromosomes. In P. thunbergii, ten strong and two weak signals appeared at the interstitial region of six pairs of the chromosomes. The hybridization sites coincided with the interstitial chromomycin A3-bands or secondary constricted regions.
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松树、密松和云杉染色体中rRNA基因的多位点分析
采用生物素标记的18S-25S rDNA原位杂交技术,在日本赤松(Pinus densiflora)和日本黑松(P. thunbergii)体细胞染色体上定位了核糖体RNA (rRNA)基因。杂交探针的细胞化学检测显示,两种植物的间期细胞核、前期和中期染色体中存在许多信号。中期补体中,这些信号在7对染色体的间隙区单独出现。在6对染色体间区出现了10个强信号和2个弱信号。杂交位点与间质染色质a3带或次生收缩区重合。
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