Isolation and partial purification of cadmium-binding components from fruiting bodies of Agaricus bisporus

Abstract

Cadmium speciation in the commercially important species Agaricus bisporus was investigated by comparing the binding of Cd in the cytosolic fractions obtained from fruiting bodies containing < 1 and 70 mg Cd kg⁻¹ (dry weight). Three Cd-binding complexes which are constitutive were isolated and partially characterised. Gel filtration, ion exchange and affinity chromatography were used in the purification procedure. Metallothionein-like components were not detected.