Colorimetric Assay Of Naproxen Tablets by Derivatization Using 4-Carboxyl-2,6-Dinitrobenzene Diazonium Ion

The East and Central African Journal of Pharmaceutical Sciences Pub Date : 2009-01-01 DOI:10.4314/ECAJPS.V12I0

S. Idowu, O. Adegoke, A. Adeniji, A. Olaniyi

{"title":"Colorimetric Assay Of Naproxen Tablets by Derivatization Using 4-Carboxyl-2,6-Dinitrobenzene Diazonium Ion","authors":"S. Idowu, O. Adegoke, A. Adeniji, A. Olaniyi","doi":"10.4314/ECAJPS.V12I0","DOIUrl":null,"url":null,"abstract":"A colorimetric assay of naproxen tablets based on chemical derivatization with the chromogenic reagent 4-carboxyl-2,6-dinitrobenzene diazonium ion was developed. The optimal reaction time was found to be 5 min at 30 o C after vortex mixing of the drug/reagent mixture for 10 s. The optimal analytical wavelength was found to be 470 nm and linearity of response was obtained between 1-7 μgml -1 of naproxen. The diazo coupling reaction occurred in a 2:1 reagent-drug stoichiometric ratio. The method is sensitive, accurate (mean recovery 101.06±3.06 %), reproducible (imprecision 3.03 % CV) and has a low limit of detection (0.95 μgm -1 ). It was applied in the assay of naproxen tablets with equivalent accuracy (p>0.05) to the official (BP) UV spectrophotometric method but combined the advantages of speed and more affordable instrumentation. The method can be applied in the in-process quality control of naproxen tablets. Keywords: Naproxen assay, 4-carboxyl-2,6-dintrobenzene diazonium ion (CDNBD), colorimetry, diazo coupling reaction East and Central African Journal of Pharmaceutical Sciences Vol. 12 (2009) 8-14","PeriodicalId":22387,"journal":{"name":"The East and Central African Journal of Pharmaceutical Sciences","volume":"20 1","pages":"8-14"},"PeriodicalIF":0.0000,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The East and Central African Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/ECAJPS.V12I0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 6

Abstract

A colorimetric assay of naproxen tablets based on chemical derivatization with the chromogenic reagent 4-carboxyl-2,6-dinitrobenzene diazonium ion was developed. The optimal reaction time was found to be 5 min at 30 o C after vortex mixing of the drug/reagent mixture for 10 s. The optimal analytical wavelength was found to be 470 nm and linearity of response was obtained between 1-7 μgml -1 of naproxen. The diazo coupling reaction occurred in a 2:1 reagent-drug stoichiometric ratio. The method is sensitive, accurate (mean recovery 101.06±3.06 %), reproducible (imprecision 3.03 % CV) and has a low limit of detection (0.95 μgm -1 ). It was applied in the assay of naproxen tablets with equivalent accuracy (p>0.05) to the official (BP) UV spectrophotometric method but combined the advantages of speed and more affordable instrumentation. The method can be applied in the in-process quality control of naproxen tablets. Keywords: Naproxen assay, 4-carboxyl-2,6-dintrobenzene diazonium ion (CDNBD), colorimetry, diazo coupling reaction East and Central African Journal of Pharmaceutical Sciences Vol. 12 (2009) 8-14

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

4-羧基-2,6-二硝基苯重氮离子衍生比色法测定萘普生片的含量

以4-羧基-2,6-二硝基苯重氮离子为显色试剂，建立了萘普生片的化学衍生比色法。药物/试剂混合物涡旋混合10 s后，最佳反应时间为30℃下5 min。最佳分析波长为470 nm，在萘普生浓度1 ~ 7 μgml -1范围内具有良好的线性关系。重氮偶联反应以2:1的试剂-药物化学计量比进行。该方法灵敏度高，准确度高(平均加样回收率为101.06±3.06%)，重复性好(不精密度为3.03% CV)，检出限低(0.95 μgm -1)。将该方法应用于萘普生片的含量测定，其准确度与常规(BP)紫外分光光度法相当(p>0.05)，且具有速度快、仪器价格便宜等优点。该方法可用于萘普生片的制程质量控制。关键词:萘普生测定，4-羧基-2,6-二硝基苯重氮离子(CDNBD)，比色法，重氮偶联反应，东非和中非药学杂志，Vol. 12 (2009) 8-14

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

The East and Central African Journal of Pharmaceutical Sciences

自引率

0.00%

发文量