Use of Isozymes to Characterize Triticum aestivum — Aegilops markgrafii Addition Lines

Abstract

Sixteen leaf and three seed isozymes were tested for their ability to identify alien chromosomes in Triticum aestivum — Aegilops markgrafii amphiploid and six descending disomic addition lines. Four leaf isozymes (NADP-dependent aromatic alcohol dehydrogenase, aspartate aminotransferase, esterase, leucine aminopeptidase) and three seed enzymes (alcohol dehydrogenase, (ß-amylase, esterase) from Ae. markgrafii were found to be informative in differentiating both the amphiploid and five of the six different addition lines from wheat. Leaf peroxidase, an eighth informative marker among the 19 isozyme systems tested was only in the amphiploid present, none of the six addition lines showed the Aegilops-specific band.