A Major Substrate for MPF: cDNA Cloning and Expression of Polypeptide Chain Elongation Factor 1γ from Goldfish ( Carassius auratus )

M. Tokumoto, Y. Nagahama, T. Tokumoto
{"title":"A Major Substrate for MPF: cDNA Cloning and Expression of Polypeptide Chain Elongation Factor 1γ from Goldfish ( Carassius auratus )","authors":"M. Tokumoto, Y. Nagahama, T. Tokumoto","doi":"10.1080/10425170290019865","DOIUrl":null,"url":null,"abstract":"One of the eukaryotic polypeptide chain elongation factors, EF-1 g n i complex, is involved in polypeptide chain elongation via the GDP/GTP exchange activity of EF-1 f. In the complex, EF-1 n has been reported to be a major substrate for maturation promoting factor (MPF). Here, we present the cloning, sequencing and expression analysis of goldfish, Carassius auratus, EF-1 n from the goldfish ovary. The cloned cDNA was 1490 u bp in length and encoded 442 amino acids. The deduced amino acid sequence was highly homologous to EF-1 n from other species. Although, the phosphorylation site identified in Xenopus EF-1 n was not conserved in the goldfish homologue, phosphorylation analysis showed that the goldfish EF-1 n was phosphorylated by MPF. We concluded that EF-1 n is a substrate for MPF during oocyte maturation in goldfish.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"1 1","pages":"27 - 31"},"PeriodicalIF":0.0000,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA Sequence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10425170290019865","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15

Abstract

One of the eukaryotic polypeptide chain elongation factors, EF-1 g n i complex, is involved in polypeptide chain elongation via the GDP/GTP exchange activity of EF-1 f. In the complex, EF-1 n has been reported to be a major substrate for maturation promoting factor (MPF). Here, we present the cloning, sequencing and expression analysis of goldfish, Carassius auratus, EF-1 n from the goldfish ovary. The cloned cDNA was 1490 u bp in length and encoded 442 amino acids. The deduced amino acid sequence was highly homologous to EF-1 n from other species. Although, the phosphorylation site identified in Xenopus EF-1 n was not conserved in the goldfish homologue, phosphorylation analysis showed that the goldfish EF-1 n was phosphorylated by MPF. We concluded that EF-1 n is a substrate for MPF during oocyte maturation in goldfish.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
MPF的主要底物:金鱼多肽链延伸因子1γ cDNA的克隆与表达
真核生物多肽链延伸因子之一EF-1 g - n复合物通过EF-1 f的GDP/GTP交换活性参与多肽链延伸。在该复合物中,EF-1 n已被报道为成熟促进因子(MPF)的主要底物。本文报道了金鱼、鲫鱼、EF-1 n的克隆、测序和表达分析。克隆的cDNA全长1490 u bp,编码442个氨基酸。推导出的氨基酸序列与其他物种的EF-1 n高度同源。虽然在爪蟾EF-1 n中发现的磷酸化位点在金鱼同源物中并不保守,但磷酸化分析表明,金鱼EF-1 n被MPF磷酸化。我们认为EF-1 n是金鱼卵母细胞成熟过程中MPF的底物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Complete mitochondrial DNA sequence of the yellowfin seabream Acanthopagrus latus and a genomic comparison among closely related sparid species Mitochondrial DNA inaugural issue Comparison of the mitochondrial genomes of East Asian Pseudolabrus fishes Content index A comprehensive analysis of three Asiatic black bear mitochondrial genomes (subspecies ussuricus, formosanus and mupinensis), with emphasis on the complete mtDNA sequence of Ursus thibetanus ussuricus (Ursidae)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1