An in vitro procedure for the estimation of in vivo digestibility of roughage plant cell wall components in herbivores using mixed rumen microorganisms

Agriculture and Environment Pub Date : 1981-11-01 DOI:10.1016/0304-1131(81)90010-2

R.A. Prins, W.C. Cliné-Theil, A.Th. Van 'T Klooster

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引用次数: 9

Abstract

The potentially indigestible plant cell wall components in representative samples of feedstuffs and animal feces (resp. a and b, expressed as a percentage of the neutral detergent residue, NDR) can be measured after incubation of these materials with mixed rumen microorganisms for several weeks at 40°C. From these values the percentage of NDR that was digested in the animal can be calculated. Similarly, the procedure can be used to estimate the digestibility of specific cell wall carbohydrates such as cellulose and hemicellulose after analysis of acid detergent fiber (ADF) and lignin.

The validity of this procedure was tested in digestion trials with ponies and wethers fed grass hay. The digestibility coefficients for NDR hemicellulose and cellulose determined with the in vitro procedure did not differ significantly from values obtanied with the conventional in vivo digestion trial.

The in vitro procedure has been applied to measure NDR digestibility in a variety of animals such as grazing geese and several species of exotic ruminants fed a mixed diet of hay and concentrates.

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利用混合瘤胃微生物估算草食动物对粗饲料细胞壁成分的体内消化率的体外方法

有代表性的饲料和动物粪便样品中潜在难以消化的植物细胞壁成分。a和b，以中性洗涤剂残留物的百分比表示，NDR)可以在这些材料与混合瘤胃微生物在40°C下孵育数周后测量。根据这些值，可以计算出NDR在动物体内被消化的百分比。同样，该方法可用于分析酸性洗涤纤维(ADF)和木质素后估计特定细胞壁碳水化合物(如纤维素和半纤维素)的消化率。该方法的有效性已在以干草为饲料的小马和马的消化试验中得到验证。用体外方法测定的NDR半纤维素和纤维素的消化率系数与常规体内消化试验所得的值无显著差异。该体外程序已被用于测量多种动物的NDR消化率，如放牧鹅和几种饲喂干草和浓缩物混合饲料的外来反刍动物。

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