Spectrophotometric determination of ammonia nitrogen in water by flow injection analysis based on NH3- o-phthalaldehyde -Na2SO3 reaction

Ying Liang, Chunmei Yan, Qing Guo, Jin Xu, Hongzhi Hu
{"title":"Spectrophotometric determination of ammonia nitrogen in water by flow injection analysis based on NH3- o-phthalaldehyde -Na2SO3 reaction","authors":"Ying Liang,&nbsp;Chunmei Yan,&nbsp;Qing Guo,&nbsp;Jin Xu,&nbsp;Hongzhi Hu","doi":"10.1016/j.ancr.2016.10.001","DOIUrl":null,"url":null,"abstract":"<div><p>The product of the NH<sub>3</sub>- <em>o-</em>phthalaldehyde (OPA) -Na<sub>2</sub>SO<sub>3</sub> reaction is rose red at pH more than 10.4, and its maximum absorption wavelength is 550 nm. Based on this, a novel spectrophotometric method with flow injection analysis has been established to determine ammonia nitrogen in water. Experimental parameters related to the flow injection method and the reaction were optimized throughout the experiments based on univariate experimental design. The length of coil is optimized as 1.6 m for storing up standard or sample solution. The optimal value is 8.20 mL/min for loading flow rate of coloring solution. The OPA concentration, sulfite concentration and reaction pH are chosen as 1.06 g/L, 0.050 g/L and 10.80, respectively. The reaction temperature and stop flow time affects the performances of the method. The linearity range and detection limit of the proposed method are 0.100–0.700 mmol/L and 0.007 mmol/L at the reaction temperature of 55 °C and stop flow time of 340 s, respectively. The sample throughput is more than 8 h<sup>−1</sup>. Under the optimal experimental conditions, the recovery is 100.4%, 95.2%, 101.7% and 92.4% for the lake water, river water, groundwater and sewage, respectively. Two lake water samples were analyzed using both the proposed method and indophenol blue method, the results show no significant difference between these two methods. In comparison with other spectrophotometric determination method of ammonia nitrogen, the main merits of the proposed method are simplicity, reliability, reproducibility and high sample throughput.</p></div>","PeriodicalId":7819,"journal":{"name":"Analytical Chemistry Research","volume":"10 ","pages":"Pages 1-8"},"PeriodicalIF":0.0000,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ancr.2016.10.001","citationCount":"39","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214181216300301","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 39

Abstract

The product of the NH3- o-phthalaldehyde (OPA) -Na2SO3 reaction is rose red at pH more than 10.4, and its maximum absorption wavelength is 550 nm. Based on this, a novel spectrophotometric method with flow injection analysis has been established to determine ammonia nitrogen in water. Experimental parameters related to the flow injection method and the reaction were optimized throughout the experiments based on univariate experimental design. The length of coil is optimized as 1.6 m for storing up standard or sample solution. The optimal value is 8.20 mL/min for loading flow rate of coloring solution. The OPA concentration, sulfite concentration and reaction pH are chosen as 1.06 g/L, 0.050 g/L and 10.80, respectively. The reaction temperature and stop flow time affects the performances of the method. The linearity range and detection limit of the proposed method are 0.100–0.700 mmol/L and 0.007 mmol/L at the reaction temperature of 55 °C and stop flow time of 340 s, respectively. The sample throughput is more than 8 h−1. Under the optimal experimental conditions, the recovery is 100.4%, 95.2%, 101.7% and 92.4% for the lake water, river water, groundwater and sewage, respectively. Two lake water samples were analyzed using both the proposed method and indophenol blue method, the results show no significant difference between these two methods. In comparison with other spectrophotometric determination method of ammonia nitrogen, the main merits of the proposed method are simplicity, reliability, reproducibility and high sample throughput.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
NH3-邻苯二醛- na2so3反应流动注射分光光度法测定水中氨氮
NH3-邻苯二醛(OPA) - na2so3反应产物在pH > 10.4时呈玫瑰红,其最大吸收波长为550 nm。在此基础上,建立了流动注射分光光度法测定水中氨氮的新方法。基于单变量实验设计,在整个实验过程中对流动注射方法和反应相关的实验参数进行了优化。线圈长度优化为1.6 m,用于储存标准溶液或样品溶液。着色液上料流速最佳值为8.20 mL/min。OPA浓度为1.06 g/L,亚硫酸盐浓度为0.050 g/L,反应pH为10.80。反应温度和停流时间影响该方法的性能。在反应温度为55℃、停流时间为340 s时,该方法的线性范围为0.100 ~ 0.700 mmol/L,检出限为0.007 mmol/L。样品吞吐量大于8 h−1。在最佳试验条件下,对湖水、河水、地下水和污水的回收率分别为100.4%、95.2%、101.7%和92.4%。用该方法和吲酚蓝法对两个湖泊水样进行了分析,结果表明两种方法之间无显著差异。与其他分光光度法测定氨氮的方法相比,该方法具有简便、可靠、重现性好、样品通量高的优点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Chemically modified carbon paste ion-selective electrodes for determination of atorvastatin calcium in pharmaceutical preparations Preparation and characterization of a novel Co(II) optode based on polymer inclusion membrane Structural identification and estimation of Rosuvastatin calcium related impurities in Rosuvastatin calcium tablet dosage form Comparative sensing of aldehyde and ammonia vapours on synthetic polypyrrole-Sn(IV)arsenotungstate nanocomposite cation exchange material Nano clay Ni/NiO nanocomposite new sorbent for separation and preconcentration dibenzothiophene from crude prior to UV–vis spectrophotometery determination
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1