Optimized Biodegradation of Pulping Effluent by Microbial Combination with Statistical Experimental Design

Honglei Chen, Yuancai Chen, H. Zhan, S. Fu
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引用次数: 1

Abstract

Statistics based experimental designs were used to construct a mixed-culture community for maximal chemical oxygen demand (COD) degradation of pulping effluents. By applying a fractional factorial design (FFD) of experiments, the significant effect of each strain on COD degradation was first quantified. Then steepest ascent method was employed to approach the experimental design space, followed by an application of response surface methodology (RSM) for further optimization. A quadratic model was found to be fit COD removal efficiency. Response surface analysis revealed that the optimum levels of the tested variables for the degradation of COD. A COD removal efficiency of (65.3 ±± 0.5) % was observed in verification experiment, which was close to the predicted value.
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利用统计实验设计优化微生物组合降解制浆废水
采用基于统计学的实验设计,构建了一个混合培养群落,用于最大化学需氧量(COD)降解制浆废水。通过实验的分数因子设计(FFD),首先量化了各菌株对COD降解的显著影响。然后采用最陡爬坡法逼近试验设计空间,并应用响应面法(RSM)进行进一步优化。建立了适合COD去除率的二次模型。响应面分析表明,各被试变量对COD降解的最佳水平。验证实验的COD去除率为(65.3±±0.5)%,与预测值接近。
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