Oleic acid improves the conidial production and quality of Metarhizium rileyi as a biocontrol agent

IF 1.5 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biocontrol Science and Technology Pub Date : 2023-06-27 DOI:10.1080/09583157.2023.2229972

Rui Xue, Guangzu Du, Cong Chen, Bin Chen, Yuejin Peng

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Abstract

ABSTRACT Metarhizium rileyi is a biological agent used in the control of various pests, particularly noctuid insects. However, this fungal species is slow-growing, resulting in a prolonged culture period and limiting its commercial production. In this study, 0.3% (w/w) oleic acid significantly enhanced the rates of hyphal growth, conidial production, and conidial germination by 23%, 9%, and 10%, respectively. The expression of genes related to microbial growth and development (MrPbs2, MrMsn2, MrSwi6, MrNsdD, Mrap1 and MrSte12) was significantly increased in the presence of oleic acid. The M. rileyi conidial production and its effects on the mortality of Spodoptera frugiperda were investigated using rice-vegetable oil solid medium (RVOSM) and its substitute Sabouraud maltose agar plus yeast extract (SMAY) plates containing 0.03% oleic acid. The fungi retained their toxicity to S. frugiperda. Therefore, oleic acid can improve the growth potential of fungi, and RVOSM can be used as a substrate for conidia production for the application of M. rileyi as a biocontrol agent.

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油酸作为一种生物防治剂，可提高栗僵菌分生孢子的产量和品质

摘要黑僵菌是一种用于防治各种害虫的生物制剂，尤其是夜蛾类昆虫。然而，这种真菌生长缓慢，导致培养周期延长，限制了其商业生产。在本研究中，0.3% (w/w)油酸能显著提高菌丝生长、分生孢子产生和孢子萌发率，分别提高23%、9%和10%。油酸的存在显著增加了微生物生长发育相关基因MrPbs2、MrMsn2、MrSwi6、MrNsdD、Mrap1和MrSte12的表达。采用含0.03%油酸的米-植物油固体培养基(RVOSM)及其替代品Sabouraud麦芽糖琼脂加酵母膏(SMAY)培养皿，研究了米-植物油固体培养基(RVOSM)对米-植物油固体培养基中米-麦芽糖琼脂加酵母膏(SMAY)的分生孢子产量及其对果斑蛾(Spodoptera frugiperda)死亡率的影响。这些真菌对水杨桃的毒力保持不变。因此，油酸可以提高真菌的生长潜力，RVOSM可以作为生分生孢子的底物，用于雷氏霉作为生物防治剂的应用。

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来源期刊

Biocontrol Science and Technology 生物-昆虫学

CiteScore

3.20

自引率

7.10%

发文量

审稿时长

4-8 weeks

期刊介绍： Biocontrol Science and Technology presents original research and reviews in the fields of biological pest, disease and weed control. The journal covers the following areas: Animal pest control by natural enemies Biocontrol of plant diseases Weed biocontrol ''Classical'' biocontrol Augmentative releases of natural enemies Quality control of beneficial organisms Microbial pesticides Properties of biocontrol agents, modes of actions and methods of application Physiology and behaviour of biocontrol agents and their interaction with hosts Pest and natural enemy dynamics, and simulation modelling Genetic improvement of natural enemies including genetic manipulation Natural enemy production, formulation, distribution and release methods Environmental impact studies Releases of selected and/or genetically manipulated organisms Safety testing The role of biocontrol methods in integrated crop protection Conservation and enhancement of natural enemy populations Effects of pesticides on biocontrol organisms Biocontrol legislation and policy, registration and commercialization.