An Investigation into the Effect of the Nitric Oxide Synthase Antagonist L-NAME and Plant Extracts on the Irritability and Barrier Function of the Skin

A. Ormerod, P. Woo, J. Islam, J. Moran, M. Cals-Grierson
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引用次数: 1

Abstract

Background: Nitric oxide (NO) synthesis is upregulated in dermatitis and may be involved in the modulation of keratinocyte proliferation and formation of the cornified envelope. Objective: To examine the possible effects of modulators of NO production on erythema and transepidermal water loss (TEWL) induced by sodium lauryl sulphate (SLS). Methods: Ten volunteers were patch tested with water and SLS 0.2, 0.5 and 1%. The outcomes of TEWL and erythema were assessed at 1, 6, 25 and 49 h. The effects of the NO inhibitors, L-NAME, extracts of Olea europa and Ginkgo biloba (as 1% gel solutions) and of the NO donor, glyceryl trinitrate (as 2% ointment) were evaluated. Results: L-NAME and the plant extracts reduced TEWL. The TEWL induced by 0.5% SLS was significantly diminished by L-NAME at 1 and 6 h and by ginkgo extract at 6 and 49 h. Erythema was too mild for reflectance measurements to show significant inhibition, but erythema scores tended to be lower at 6 h the application of NO inhibitors. Conclusion: NO inhibitors may protect skin barrier function from the adverse effects of chemical irritants.
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一氧化氮合酶拮抗剂L-NAME与植物提取物对皮肤过敏性和屏障功能影响的研究
背景:一氧化氮(NO)合成在皮炎中上调,可能参与角化细胞增殖和角化包膜形成的调节。目的:探讨一氧化氮调节剂对十二烷基硫酸钠(SLS)所致红斑和经皮失水(TEWL)的影响。方法:10名志愿者分别用水、0.2、0.5、1%的SLS贴片试验。在第1、6、25和49小时评估TEWL和红斑的结果。评估NO抑制剂L-NAME、油橄榄和银杏提取物(作为1%凝胶溶液)和NO供体三硝酸甘油(作为2%软膏)的效果。结果:L-NAME和植物提取物均能降低TEWL。0.5% SLS诱导的TEWL在1和6 h时被L-NAME显著降低,在6和49 h时被银杏提取物显著降低。红斑过于轻微,无法进行反射测量,但在使用NO抑制剂6 h时,红斑评分往往较低。结论:一氧化氮抑制剂可保护皮肤屏障功能免受化学刺激物的不良反应。
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Acknowledgement to Referees for Exogenous Dermatology 2004 Contents Vol. 3, 2004 Author Index Vol. 3, 2004 Is There Evidence that Geraniol Causes Allergic Contact Dermatitis? Subject Index Vol. 3, 2004
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