The evidence for open and closed exocytosis as the primary release mechanism

IF 7.2 2区生物学 Q1 BIOPHYSICS Quarterly Reviews of Biophysics Pub Date : 2016-07-18 DOI:10.1017/S0033583516000081

Lin Ren, Lisa J. Mellander, Jacqueline D. Keighron, A. Cans, M. Kurczy, I. Svir, A. Oleinick, C. Amatore, A. Ewing

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引用次数: 59

Abstract

Abstract Exocytosis is the fundamental process by which cells communicate with each other. The events that lead up to the fusion of a vesicle loaded with chemical messenger with the cell membrane were the subject of a Nobel Prize in 2013. However, the processes occurring after the initial formation of a fusion pore are very much still in debate. The release of chemical messenger has traditionally been thought to occur through full distention of the vesicle membrane, hence assuming exocytosis to be all or none. In contrast to the all or none hypothesis, here we discuss the evidence that during exocytosis the vesicle-membrane pore opens to release only a portion of the transmitter content during exocytosis and then close again. This open and closed exocytosis is distinct from kiss-and-run exocytosis, in that it appears to be the main content released during regular exocytosis. The evidence for this partial release via open and closed exocytosis is presented considering primarily the quantitative evidence obtained with amperometry.

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证据表明开放和封闭胞吐是主要的释放机制

胞吐是细胞相互沟通的基本过程。导致装载化学信使的囊泡与细胞膜融合的事件是2013年诺贝尔奖的主题。然而，融合孔初始形成后发生的过程仍然存在很大的争议。化学信使的释放传统上被认为是通过囊泡膜的完全膨胀发生的，因此假设胞吐是全部或没有。与全或无假说相反，我们在这里讨论的证据表明，在胞吐过程中，囊泡膜孔打开仅释放一部分递质内容物，然后再次关闭。这种开放和封闭的胞吐不同于吻跑胞吐，因为它似乎是正常胞吐过程中释放的主要内容。通过开放和封闭的胞吐作用部分释放的证据主要考虑了用安培法获得的定量证据。

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来源期刊

Quarterly Reviews of Biophysics 生物-生物物理

CiteScore

12.90

自引率

1.60%

发文量

期刊介绍： Quarterly Reviews of Biophysics covers the field of experimental and computational biophysics. Experimental biophysics span across different physics-based measurements such as optical microscopy, super-resolution imaging, electron microscopy, X-ray and neutron diffraction, spectroscopy, calorimetry, thermodynamics and their integrated uses. Computational biophysics includes theory, simulations, bioinformatics and system analysis. These biophysical methodologies are used to discover the structure, function and physiology of biological systems in varying complexities from cells, organelles, membranes, protein-nucleic acid complexes, molecular machines to molecules. The majority of reviews published are invited from authors who have made significant contributions to the field, who give critical, readable and sometimes controversial accounts of recent progress and problems in their specialty. The journal has long-standing, worldwide reputation, demonstrated by its high ranking in the ISI Science Citation Index, as a forum for general and specialized communication between biophysicists working in different areas. Thematic issues are occasionally published.