Examination of method for the measurement of acetaldehyde in human plasma by using 9,10-phenanthrenequinone as a derivatizing agent

Abstract

Blood acetaldehyde levels are often measured to elucidate individual diŠerences and pharmacokinetics of alcohol metabolism due to the gene polymorphisms of aldehyde ship ( R 2 ＝ 0.9994 ) in the range of 3  100 m M indicated strong results. The limit of detection and quantiˆcation was 1 m M and 3 m M, respectively. The intra- and inter-day precision were both below 13.0  and accuracy was in the range of － 8.5  to 2.5  . The extraction recoveries of acetaldehyde in human plasma ranged from 80.4  to 106.8  . The derivative was stable at 4 ° C for 2 days. When acetaldehyde was added in human plasma and derivatized with PQ, acetaldehyde could be quantiˆed by liquid chromatography-mass spectrometry. However, after drinking alcohol, acetaldehyde was measured in healthy subjects with ALDH2  1 / 2 , but not with ALDH2  1 /  1 . Acetaldehyde was di‹cult to detect in subjects with ALDH2  1 / 1 , as they are rapid metabolizers of acetaldehyde resulting in low concentrations of acetaldehyde. Acetaldehyde was detected in those subjects with ALDH2  1 / 2 , however, the measured acetaldehyde levels varied between the analytical methods.