Experimental study on the interaction of insulin with apatitic calcium phosphates analogous to bone mineral: adsorption and release

Q2 Chemistry Current Chemistry Letters Pub Date : 2022-01-01 DOI:10.5267/j.ccl.2022.6.003

A. E. Rhilassi, M. Bennani-Ziatni

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Abstract

The present work investigated the interaction of human insulin with synthesized poorly crystalline apatitic calcium phosphates containing simultaneously HPO42- and CO32- ions in various amount. The adsorption kinetics is very fast, while the release kinetics is generally slow. The chemical composition of apatite has an influence on both the adsorption and release processes. The experimental results show that the percentage of insulin adsorption and release decreased with the increase of the content of carbonate. The equilibrium adsorption data are fitted into Langmuir, Freundlich, Elovich, Temkin, and Dubinin–Radushkevich isotherms. The Langmuir model is best suited with a maximum monolayer adsorption capacity of 33.20 and 25.08 mg/g at 310 K corresponding to the carbonated and octocalcium phosphate apatite respectively. Isotherms parameters have revealed that the adsorption of insulin on these apatites is a feasible, spontaneous, and exothermic process. Fourier-transforms infrared confirm the fixation of insulin on non-carbonated and carbonated apatite. The adsorption and release of insulin molecules can be well described as an ions exchange-reaction between species in the hydrated layer of apatite and other species in solution. All of these results suggested that apatitic calcium phosphates can be used as systems for insulin delivery.

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胰岛素与类似骨矿物质的磷灰质磷酸钙相互作用的实验研究:吸附与释放

本文研究了人胰岛素与合成的同时含有不同量HPO42-和CO32-离子的低结晶磷灰石磷酸钙的相互作用。吸附动力学非常快，而释放动力学一般较慢。磷灰石的化学成分对吸附和释放过程都有影响。实验结果表明，胰岛素的吸附和释放率随碳酸盐含量的增加而降低。平衡吸附数据拟合到Langmuir、Freundlich、Elovich、Temkin和Dubinin-Radushkevich等温线上。Langmuir模型最适合于碳酸磷灰石和磷酸八钙磷灰石在310 K时的最大单层吸附容量分别为33.20和25.08 mg/g。等温线参数显示，胰岛素在这些磷灰石上的吸附是一个可行的、自发的、放热的过程。傅里叶红外变换证实了胰岛素在非碳化和碳化磷灰石上的固定作用。胰岛素分子的吸附和释放可以很好地描述为磷灰石水合层中的离子与溶液中其他离子之间的交换反应。这些结果表明，磷灰石磷酸钙可以作为胰岛素递送系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Chemistry Letters Chemistry-Chemistry (all)

CiteScore

4.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： The "Current Chemistry Letters" is a peer-reviewed international journal which aims to publish all the current and outstanding research articles, reviews and letters in chemistry including analytical chemistry, green chemistry, inorganic chemistry, organic chemistry, physical chemistry, etc. This journal is dedicated to serve all academic and industrial researchers and scientists who are expert in all major advances in chemistry research. The journal aims to provide the most complete and reliable source of information on current developments in these fields. The emphasis will be on publishing quality articles rapidly and openly available to researchers worldwide. Please note readers are free to read, download, copy, distribute, print, search, or link to the full texts of articles published on this journal. Current Chemistry Letters is an open access journal, which provides instant access to the full text of research papers without any need for a subscription to the journal where the papers are published. Therefore, anyone has the opportunity to copy, use, redistribute, transmit/display the work publicly and to distribute derivative works, in any sort of digital form for any responsible purpose, subject to appropriate attribution of authorship. Authors who publish their articles may also maintain the copyright of their articles.