Anti-Microbial Activities and Phytochemical Screening of the Premna Odorata Blanco Alagaw Leaf Extract

Abstract

Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/ by/4.0) ABSTRACT The researcher focused mainly to determine the antimicrobial properties and phytochemical screening of the Alagaw leaf extract. Specifically, this study was conducted to determine the percent yield, antimicrobial activities and the secondary metabolites of Alagaw leaf extract which was analyzed and it include alkaloid, anthraquinone, saponins and steroid. Findings of the study showed that the Alagaw leaf extract has a percent yield of 11.5%. Anti-Microbial Activity was tested by petri disk on a plate Nutrient Agar streaked with the E. coli bacteria, the plates were incubated for 24hrs and 37oC. Results were observed for the presence of zone inhibition (clear area) around the test disk. Results from this study showed that the antimicrobial activity on E. coli as indicated as negative by the presence of Alagaw leaf extract. Furthermore, the result suggests that the Alagaw leaf extract did not suppress the growth of the E. coli bacteria, hence it indicates that it has no anti-microbial effect to the test organism. While the secondary metabolites such as alkaloid, anthraquinone, saponins and steroid is found negative. It is therefore recommended that further study of the chemical properties of alagaw leaf extract, barks and roots should be conducted