Cytotoxic activity and antioxidant effects of Origanum onites essential oil and its two major contents, carvacrol and p-cymene on human colorectal (HCT116) and hepatocelluler carcinoma (HepG2) cell lines

IF 2.2 3区农林科学 Q3 CHEMISTRY, APPLIED Journal of Essential Oil Research Pub Date : 2022-08-11 DOI:10.1080/10412905.2022.2107101

E. Becer, E. M. Altundag, K. Başer, H. Vatansever, M. Bayar

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引用次数: 9

Abstract

ABSTRACT Origanum onites L. (O. onites) is one of the cultivated Origanum species in Turkey. The aim of this study was to determine the cytotoxicity and cellular antioxidant effects of O. onites essential oil, carvacrol and p-cymene on HCT116 and HepG2 cells. O. onites essential oil components were analyzed by GC/MS and GC/FID. Cytotoxicity was analyzed via the MTT assay in HCT116 and HepG2 cells. Cellular antioxidant activities of O. onites essential oil, carvacrol and p-cymene were determined with DCFH-DA method. GC/MS analysis identified carvacrol (%78.4) as the main constituent of O. onites essential oil. 400 µg/ml concentration of O. onites essential oil, carvacrol and p-cymene were found to be active against HCT116 and HepG2 cells. Also, carvacrol showed higher cellular antioxidant activity than O. onites essential oil and p-cymene. We conclude that O. onites essential oil and carvacrol may be a candidate for pharmaceutical products in treatment of cancer.

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土豆泥精油及其两种主要成分香芹酚和对伞花素对人大肠癌(HCT116)和肝癌(HepG2)细胞的细胞毒活性和抗氧化作用

土一枝(Origanum onites L.， O. onites)是土耳其的一种人工培育的土一枝。本研究的目的是研究石杉精油、香芹酚和对花香烃对HCT116和HepG2细胞的细胞毒性和细胞抗氧化作用。采用GC/MS、GC/FID等分析方法对黄芪挥发油成分进行了分析。MTT法检测HCT116和HepG2细胞的细胞毒性。采用DCFH-DA法测定了龙葵挥发油、香芹酚和对花香烃的细胞抗氧化活性。GC/MS分析鉴定出香芹酚(% 78.4%)为龙葵挥发油的主要成分。400µg/ml浓度的龙葵精油、香芹酚和对花香烃对HCT116和HepG2细胞均有抑制作用。此外，香芹酚的细胞抗氧化活性也高于石楠精油和对花香烃。我们得出结论，龙葵精油和香芹酚可能是治疗癌症的候选药物产品。

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来源期刊

Journal of Essential Oil Research 工程技术-食品科技

CiteScore

6.00

自引率

3.30%

发文量

审稿时长

18-36 weeks

期刊介绍： Journal of Essential Oil Research ( JEOR) is the major forum for the publication of essential oil research and analysis. Each issue includes studies performed on the chemical composition of some of the 20,000 aromatic plants known in the plant kingdom. JEOR is devoted entirely to all phases of research from every corner of the world by the experts in their field. JEOR''s main areas of focus include: -Analytical chemistry- Biological activity- Biotechnology- Chemical composition- Chemical synthesis- Chemosystematics- Microbiological activity- Plant biochemistry/biosynthesis- Toxicology. Published six times per year, JEOR provides articles on the aromatic principles of a plant or its isolates and are directed toward furthering our readers'' knowledge of the aromatic plant and animal kingdoms.