Optimization of Chaetoceros gracilis Microalgae Production for Fish Feeding Using an Airlift Photobioreactor

Oscar R. Pupo, Samira Garcia, L. Maré, S. Gómez, A. Bula
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Abstract

An experimental procedure was carried out to maximize Chaetoceros gracilis growth. Chaetoceros gracilis , marine microalgae, is considered for feeding fisheries with no GMO (Genetically Modified Organisms) to avoid human health hazards. Furthermore, following United Nations Resolution on water, the microalgae is grown in photobioreactors due to its low water usage. To maximize the microalgae growth, an experimental design was carried out to analyze the effects of Light Intensity, CO 2 supply per day, Sparger type, Photoperiod and Inlet airflow, pH and water temperature were monitored but not controlled. It was found that Light intensity and CO 2 supply per day have statistical significance. Out of three possible scenarios, 1700 lux and 80 gr/day of CO 2 , leads to a cell density at day three of 310×10 4 cel/mL which represents 20% more of the density attained in day two under bag (standard) growing conditions. It was also found that water Ph has also a strong effect over cell density.
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气升式光生物反应器生产微藻的优化研究
为了使薄壁毛藻的生长最大化,进行了一种实验程序。为避免对人类健康造成危害,考虑将微藻毛羽(Chaetoceros gracilis)用于不含转基因生物的渔业。此外,根据联合国关于水的决议,由于微藻的用水量低,因此在光生物反应器中生长。为了最大限度地促进微藻生长,进行了试验设计,分析了光照强度、CO 2日供给量、Sparger类型、光周期和进水气流对微藻生长的影响,并对pH和水温进行了监测但不进行控制。光照强度和日均CO 2供给量差异有统计学意义。在三种可能的情况下,1700勒克斯和80克/天的二氧化碳,导致第三天的细胞密度为310×10 4细胞/mL,比在袋(标准)生长条件下第二天达到的密度多20%。研究还发现,水的Ph值对细胞密度也有很大的影响。
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