Hemoglobin-Modified Core–Shell Fe3O4@Au Nanostructures for the Electrochemical Detection of Acrylamid

IF 0.8 Q3 MULTIDISCIPLINARY SCIENCES Makara Journal of Science Pub Date : 2021-09-30 DOI:10.7454/mss.v25i3.1232
E. Saepudin, T. Yuliani, M. A. F. Nasution, M. Khalil, J. Hong, T. Ivandini
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引用次数: 0

Abstract

In this study, electrochemical detection of acrylamide using hemoglobin (Hb)-modified core–shell Fe3O4@Au nanostructures was conducted. Fe3O4 nanoparticles (~4.9 nm) and core–shell Fe3O4@Au (5.0–6.4 nm) nanostructures were successfully synthesized by the thermal decomposition method. Electrochemical investigation revealed that the optimum amount of Hb of 2 mg/mL could be immobilized in 0.1 M acetate buffer solution (pH = 6). Moreover, the detection of acrylamide using Fe3O4@Au/Hb was evaluated by the cyclic voltammetry technique. A linear calibration curve (R = 0.98) in the concentration range of 0.1 to 1.0 μM could be achieved with an estimated limit of detection, limit of quantification, and sensitivity of 0.136 μM, 0.453 μM, and 0.4411 μA/μM, respectively. Furthermore, the developed biosensor exhibited high selectivity in the presence of ascorbic acid, melamine, and caffeine. The developed biosensor was applied to the detection of acrylamide in coffee samples and validated using the standard high-performance liquid chromatography (HPLC) method. The concentration of acrylamide in coffee samples was determined to be 37.450 and 35.377 ppm using electrochemical measurement and HPLC, respectively.
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血红蛋白修饰核壳Fe3O4@Au纳米结构用于丙烯酰胺的电化学检测
在这项研究中,利用血红蛋白修饰的核壳Fe3O4@Au纳米结构进行了丙烯酰胺的电化学检测。采用热分解法制备了Fe3O4纳米颗粒(~4.9 nm)和核壳Fe3O4@Au (5.0 ~ 6.4 nm)纳米结构。电化学研究表明,在0.1 M醋酸缓冲溶液(pH = 6)中,Hb的最佳固定量为2 mg/mL。此外,通过循环伏安法评价了Fe3O4@Au/Hb对丙烯酰胺的检测效果。在0.1 ~ 1.0 μM浓度范围内可获得线性校准曲线(R = 0.98),估计检出限、定量限和灵敏度分别为0.136 μM、0.453 μM和0.4411 μA/μM。此外,所开发的生物传感器在抗坏血酸、三聚氰胺和咖啡因的存在下表现出高选择性。将所研制的生物传感器应用于咖啡样品中丙烯酰胺的检测,并采用标准的高效液相色谱(HPLC)方法进行验证。用电化学法和高效液相色谱法测定了咖啡样品中丙烯酰胺的浓度分别为37.450和35.377 ppm。
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来源期刊
Makara Journal of Science
Makara Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.30
自引率
20.00%
发文量
24
审稿时长
24 weeks
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