ISOENZYMES AND ACTIVITY OF LACCASES PRODUCED BY Pleurotus ostreatus GROWN AT DIFFERENT TEMPERATURES

Abstract

Pleurotus ostreatus produces laccase isoenzymes depending on the conditions in which it grows, it has been suggested that the presence of inducers, the pH of the culture medium as well as the type and concentration of carbon and nitrogen sources promote the production of these isoenzymes, however, the e ff ect of temperature of Pleurotus ostreatus growth on its production of laccase isoenzymes has not been evaluated. In this study, Pleurotus ostreatus was grown in solid-state fermentation at di ff erent temperatures (10, 15, 20, 25, 30 and 35 °C) and the activity and isoenzymes number of laccases produced in each bioprocess was determined. At 10 and 35 °C there was no growth of the fungus. The activity of laccases was di ff erent in each fermentation, being at 25 ºC where the highest value was observed (20000 U L − 1 ). The laccase isoenzymes pattern was also di ff erent depending on the incubation temperature of the fungus, it was observed in some times of the culture, 4 isoenzymes in the fermentations incubated at 15 and 20 ºC, 3 isoenzymes at 25 ºC and 2 isoenzymes at 30 ºC. These results show that the growth temperature of Pleurotus ostreatus modifies its activity profiles and its isoenzyme patterns of laccases. Pleurotus , solid-state fermentation, temperature, zymograms.