Ozone Impacts in Suppressing Stored Grain Insect Pests in Maize: A Clean and Safe Control Strategy Compared with Phosphine Toxin-Gases

Esther Martínez-Peralta, A. Ahmed, M. Cabezas-Guerrero
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Abstract

ha) However, is destined production of balanced feed. Maize cultivars are vulnerable to an invasion of various primary and secondary insect pests, negatively influence the ABSTRACT The study was conducted under greenhouse (Temp. between 27 - 32 ºC and relative humidity 65%) and laboratory conditions of the Industrial Complex of the Equaquimic Company, located in the city of Quevedo province Los Ríos, at km. 4.5 via Quevedo – Valencia, coordinates 0°58'57.1" S and 79°26'28.9"W. to evaluate the mortality percentages of 20 ppm ozone dose under different exposure times (30, 45 and 60 minutes) on three of the most dangerous stored grain insect pests: Sitophilus zeamais (Motschulsky), Rhyzopertha dominica (Fabricius) and Tribolium castaneum (Herbst); separately for each insect with corn grains and without grains by direct exposure to each pest. Three experiments were performed, one for each insect species in a Complete Randomized Design (RCBD) in factorial arrangement (Time and condition). The results indicated that there were no significant differences in the interaction for insect mortality. The mortality was significantly affected by exposure time when corn was used or not. The mortality of ozone under 60 minutes of the exposure was recorded (< 90%), while 30 minutes, the shortest exposure time, showed average mortality of 81%; respectively for each insect species. In general, the average mortality in all exposure periods, with corn was 81%, while without corn was 98%for each insect species. These results demonstrate the efficacy of ozone as an alternative viable tool to control - stored grain insects.
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臭氧对玉米储粮害虫的抑制作用:与磷化氢毒素气体比较的清洁安全控制策略
ha)然而,注定要生产平衡饲料。摘要本研究是在温室(温度27 - 32℃,相对湿度65%)和实验室条件下进行的,实验条件为Equaquimic公司位于克维多省Los Ríos市的工业园区,距离km。4.5经克维多-瓦伦西亚,坐标0°58'57.1" S和79°26'28.9"W。评估20 ppm臭氧剂量在不同暴露时间(30、45和60分钟)下对玉米象(motsschulsky)、灰蛾(Fabricius)和castaneum (Herbst) 3种最危险的储粮害虫的死亡率百分比;分别对每一种有玉米粒和没有玉米粒的昆虫进行直接接触。采用完全随机设计(RCBD),按因子安排(时间和条件)对每一种昆虫进行3个试验。结果表明,对昆虫死亡率的互作无显著差异。施用玉米和不施用玉米的暴露时间对死亡率有显著影响。臭氧暴露60分钟内死亡率< 90%,暴露时间最短的30分钟平均死亡率为81%;分别为每种昆虫。各虫种在各暴露期的平均死亡率,有玉米时为81%,无玉米时为98%。这些结果表明,臭氧作为一种可行的替代手段来控制储粮害虫的有效性。
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