DNA Barcoding and Intra Species Analysis of the Ember Parrot Fish Scarus Rubroviolaceus using mtCO1

Abstract

In this study S.rubroviolaceus form very ancient family group Scorpaenidae with structurally diverse group of subphylum Vertebrata was collected form from parangipettai (MAB06)for species identification with inter specific variation among barcoding region sequenceing cytochrome oxidase subunit I ( COI) gene comparing with Australia (EF609452), Tonga (FJ584082), South Africa (GU805008), Iran (HQ149928). The intraspecific variation analysis in NCBI screend for COI sequence form different geographical regions results 99% to 100% identity.  BOLD’s identification engine shows 99.68% to 100% similar of 20 specimens with conformation of  COI species database tree that S.rubroviolaceus belongs to order Carangidae. The molar concentration of nucleotides in the COI region profiling barcoding valuates that high in thymine content are similar in all the samples, Cytosine as second predominant and guanine as least molar concentration. A helical wheel is ploted to illustrate the properties of alpha helices in proteins. Multiple sequence alignments for DNA sequences similarities query were analysed with Clustal W from EMBL tools.