Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola (Strophariaceae)

Abstract

A 4 231 bp DNA fragment of B mating type pheromone receptor from strain YAASM0711 of Agrocybe salicacola was obtained by using degenerate PCR and DNA walking techniques.The result of alignment and structure prediction of DNA sequences showed that one 1 194 bp nucleotides gene ASRcb1 encoding B mating pheromone receptor was found,including four introns(72 bp,49 bp,48 bp and 41 bp) and five extrons(217 bp,113 bp,67 bp,138 bp and 449 bp).The spliced open reading frame(ORF) contains 984 bp nucleotides encoding 327 amino acid residues,and includes seven transmembrane protein regions as in its similar sequences of Coprinus cinerea and Laccaria bicolor pheromone receptors.The genetic evolution of pheromone receptor showed ASRcb1 was clustered with more receptors,which suggested multiple ways of evolution occurred in fungi.