Identification of a plant derivative (Hibiscus cannabinus) for mosquito (Anopheles darlingi) control using in silico protein-protein docking techniques

Abstract

Hibiscus cannabinus, often known as kenaf, is contain significant amount of bioactive compounds and acts as a natural antioxidant. The aim of this in silico investigation is to find out how the flower extract, Sulfite oxidase inhibits the mosquito ( Anopheles darlingi ) protein, pro-resilin, using 3D automated drug docking studies. In this methodology, we perform primary analysis on the amino acid sequences of pro-resilin and sulphite oxidase using NCBI GenPept and HDock server for performing drug docking analysis. The overall results clearly elucidate that the intramolecular dynamic interaction between Sulfite oxidase and the motif regions of Pro-resilin is very effective. This results in the downregulation of the expression of Pro-resilin which has been shown in 3D form. At present, research on mosquito control is of prime focus at global level. Our in silico research investigation is a safe initiative to control mosquitoes using flower extract as it is devoid of side effects.