Elucidation of lipopeptide biosurfactants responsible for the larvicidal activity of Bacillus velezensis PHP1601 towards Lucilia cuprina larvae

IF 1.2 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biocontrol Science and Technology Pub Date : 2023-07-02 DOI:10.1080/09583157.2023.2231180

D. R. Ramesar, C. Hunter

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引用次数: 2

Abstract

ABSTRACT Lipopeptide biosurfactant compounds derived from cultures of Bacillus velezensis PHP1601 (Bacillales: Bacillaceae) show antagonism towards the larval stage of Lucilia cuprina (Diptera: Calliphoridae), a blowfly pest of agricultural significance. A study was undertaken to characterise and elucidate the lipopeptide biosurfactant compounds contributing to this effect. Lipopeptide extracts were obtained from cultures grown in Landy medium by acid precipitation and methanol extraction. Thin layer chromatography and UPLC ESI-TOF MS were used to partially purify and characterise the lipopeptides present in the extract. Lipopeptide fractions contained homologues of surfactin (C13–C17), fengycin (C14–C17) and iturin (C14–C17). Each lipopeptide fraction (20 µg g−1) displayed larvicidal activity against second-instar L. cuprina larvae, with a highly polar surfactin fraction (Rf: 0.90) being the most effective. The potency of surfactin was confirmed with bioassays incorporating a surfactin standard whereby a LT50 of 179.97 h and LC50 of 9.87 µg g−1 was determined. Interestingly, larvae cadavers recovered from the bioassays displayed significant physiological discolouration and stunting; this was attributed to the biosurfactant nature of the lipopeptide compounds. These findings corroborate the role of lipopeptide compounds, specifically surfactin, in the fly biocontrol mechanism of PHP1601 and constitute the first report of these compounds being insecticidal towards blowfly larvae.

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velezensis芽孢杆菌PHP1601对铜绿蝇幼虫具有杀虫活性的脂肽生物表面活性剂的鉴定

摘要:从velezensis芽孢杆菌PHP1601(芽孢杆菌:芽孢杆菌科)培养物中提取的脂肽类生物表面活性剂化合物对具有重要农业意义的绿盲蝽(Lucilia cuprina)幼虫期具有拮抗作用。进行了一项研究，以表征和阐明脂肽生物表面活性剂化合物有助于这种效果。从Landy培养基中通过酸沉淀和甲醇萃取得到脂肽提取物。采用薄层色谱和UPLC ESI-TOF MS对提取物中的脂肽进行了部分纯化和表征。脂肽部分含有surfactin (C13-C17)、fengycin (C14-C17)和iturin (C14-C17)的同源物。各脂肽组分(20µg g−1)对铜夜蛾二龄幼虫均有杀虫活性，其中高极性表面素组分(Rf: 0.90)的杀虫效果最好。采用生物测定法确定了表面素的效价，其中LT50为179.97 h, LC50为9.87µg g−1。有趣的是，从生物检测中恢复的幼虫尸体显示出明显的生理变色和发育迟缓;这归因于脂肽化合物的生物表面活性剂性质。这些发现证实了脂肽化合物，特别是表面素，在PHP1601对苍蝇幼虫的生物防治机制中的作用，并首次报道了这些化合物对苍蝇幼虫的杀虫作用。

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来源期刊

Biocontrol Science and Technology 生物-昆虫学

CiteScore

3.20

自引率

7.10%

发文量

审稿时长

4-8 weeks

期刊介绍： Biocontrol Science and Technology presents original research and reviews in the fields of biological pest, disease and weed control. The journal covers the following areas: Animal pest control by natural enemies Biocontrol of plant diseases Weed biocontrol ''Classical'' biocontrol Augmentative releases of natural enemies Quality control of beneficial organisms Microbial pesticides Properties of biocontrol agents, modes of actions and methods of application Physiology and behaviour of biocontrol agents and their interaction with hosts Pest and natural enemy dynamics, and simulation modelling Genetic improvement of natural enemies including genetic manipulation Natural enemy production, formulation, distribution and release methods Environmental impact studies Releases of selected and/or genetically manipulated organisms Safety testing The role of biocontrol methods in integrated crop protection Conservation and enhancement of natural enemy populations Effects of pesticides on biocontrol organisms Biocontrol legislation and policy, registration and commercialization.